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CDC2 (phospho Tyr15) antibody

Anti-CDC2 (phospho Tyr15) antibody used in Immunoprecipitation (IP). GTX128155
Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX128155
Anti-CDC2 (phospho Tyr15) antibody used in IHC (Paraffin sections) (IHC-P). GTX128155
Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX128155

Cat No. GTX128155

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

HeLa (untreated) , HeLa (2mM double thymidine treatment and recovery for 3.5hr) , HeLa (2mM double thymidine treatment and recovery for 12hr) , HeLa (100ng/ml Nocodazole treatment for 24hr) , Rat-2 (untreated) , Rat-2 (100ng/ml Nocodazole treatment for 24

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep, Drosophila, Bovine, Chicken, Pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human CDC2 (phospho Tyr15). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin dependent kinase 1 , CDC2 , CDC28A , P34CDC2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDC2 (phospho Tyr15) antibody used in Immunoprecipitation (IP). GTX128155

GTX128155 IP Image

Immunoprecipitation of CDC2 (phospho Tyr15) protein from HeLa whole cell extracts using 5 μg of CDC2 (phospho Tyr15) antibody (GTX128155).
Western blot analysis was performed using CDC2 (phospho Tyr15) antibody (GTX128155).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX128155

GTX128155 WB Image

CDC2 (phospho Tyr15) antibody detects CDK1 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (2mM double thymidine treatment and recovery for 3.5hr)
C. 30 μg HeLa whole cell lysate/extract (2mM double thymidine treatment and recovery for 12hr)
10 % SDS-PAGE
CDC2 (phospho Tyr15) antibody (GTX128155) dilution: 1:1000

Anti-CDC2 (phospho Tyr15) antibody used in IHC (Paraffin sections) (IHC-P). GTX128155

GTX128155 IHC-P Image

CDC2 (phospho Tyr15) antibody detects CDC2 (phospho Tyr15) protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
CDC2 (phospho Tyr15) antibody (GTX128155) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX128155

GTX128155 WB Image

CDC2 (phospho Tyr15) antibody detects CDK1 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
10 % SDS-PAGE
CDC2 (phospho Tyr15) antibody (GTX128155) dilution: 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169