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CDC2 (phospho Tyr15) antibody

Orthogonal
Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX128155
Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX128155
Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX128155
Anti-CDC2 (phospho Tyr15) antibody used in Immunoprecipitation (IP). GTX128155
Anti-CDC2 (phospho Tyr15) antibody used in IHC (Paraffin sections) (IHC-P). GTX128155
Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX128155

Cat. No. GTX128155

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all CDC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

HeLa (2mM double thymidine treatment and recovery for 3.5hr) , HeLa (100ng/ml Nocodazole treatment for 24hr)

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep, Bovine, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr15 of human CDC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2877127

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 1 , CDC2 , CDC28A , P34CDC2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX128155

GTX128155 WB Image

Orthogonal

CDC2 (phospho Tyr15) antibody detects CDK1 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (2mM double thymidine treatment and recovery for 3.5hr)
C. 30 μg HeLa whole cell lysate/extract (2mM double thymidine treatment and recovery for 12hr)
10 % SDS-PAGE
CDC2 (phospho Tyr15) antibody (GTX128155) dilution: 1:1000

Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX128155

GTX128155 WB Image

Orthogonal

CDC2 (phospho Tyr15) antibody detects CDK1 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
10 % SDS-PAGE
CDC2 (phospho Tyr15) antibody (GTX128155) dilution: 1:1000

Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX128155

GTX128155 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CDC2 (phospho Tyr15) antibody (GTX128155) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CDC2 (phospho Tyr15) antibody used in Immunoprecipitation (IP). GTX128155

GTX128155 IP Image

Immunoprecipitation of CDC2 (phospho Tyr15) protein from HeLa whole cell extracts using 5 μg of CDC2 (phospho Tyr15) antibody (GTX128155).
Western blot analysis was performed using CDC2 (phospho Tyr15) antibody (GTX128155).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CDC2 (phospho Tyr15) antibody used in IHC (Paraffin sections) (IHC-P). GTX128155

GTX128155 IHC-P Image

CDC2 (phospho Tyr15) antibody detects CDC2 (phospho Tyr15) protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
CDC2 (phospho Tyr15) antibody (GTX128155) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX128155

GTX128155 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC2 (phospho Tyr15) antibody (GTX128155) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

YY Hsieh et al. Mol Oncol 2023; Repositioning VU-0365114 as a novel microtubule-destabilizing agent for treating cancer and overcoming drug resistance
CP Chen et al. Biomed Pharmacother 2023; 166 : 115389
Application : WB
Xiaobo Wang et al. J Radiat Res 2022; 63 (3) : 331-341 Overcoming radio-resistance in esophageal squamous cell carcinoma via hypermethylation of PIK3C3 promoter region mediated by KDM5B loss.
Application : WB
Reactivity : Human
Kuo TT et al. ACS Omega 2020; 5 (49) : 31792-31800 Melissa officinalis Extract Induces Apoptosis and Inhibits Migration in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Lin YW et al. Cytometry B Clin Cytom 2014; The application of flow cytometry for evaluating biological aggressiveness of intracranial meningiomas.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169