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CDC2 (phospho Tyr15) antibody

Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX50273
Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX50273
Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX50273

Cat. No. GTX50273

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all CDC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 15 (G-T-Y(p)-G-V) derived from human CDC2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin dependent kinase 1 , CDC2 , CDC28A , P34CDC2

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX50273

GTX50273 WB Image

WB analysis of extracts from HeLa cells untreated (lane 1) or treated with UV (lane 2) using GTX50273 CDC2 (phospho Tyr15) antibody.

Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX50273

GTX50273 WB Image

WB analysis of extracts from 293 cells treated with Hydroxyurea or calf intestinal phosphatase (CIP) using GTX50273 CDC2 (phospho Tyr15) antibody.

Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX50273

GTX50273 WB Image

WB analysis of extracts from 293 cells treated with Hydroxyurea or calf intestinal phosphatase (CIP) using GTX50273 CDC2 (phospho Tyr15) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399