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CDC2 (phospho Tyr15) antibody

Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX79116

Cat. No. GTX79116

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of tyrosine 15 (G-T-Yp-G-V).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11165774

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CDC2,CDC28A,CDK1,P34CDC2,cyclin dependent kinase 1,cdc2

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion,Cytoskeleton,Microtubule organizing center,Centrosome,Spindle

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX79116

GTX79116 WB Image

WB analysis of COLO (Lane 1, 5), HepG2 (Lane 2, 6), and K562 (Lane 3, 4, 7, 8) cell lysates using GTX79116 CDC2 (phospho Tyr15) antibody.
Lane 1 - 4 : CDC2 antibody
Lane 5 - 8 : GTX79116 CDC2 (phospho Tyr15) antibody

REFERENCE

There are currently no references for CDC2 (phospho Tyr15) antibody (GTX79116). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CDC2 (phospho Tyr15) antibody (GTX79116). Be the first to share your experience with this product.
Package List Price ($)
$ 399