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CDC2 (phospho Tyr15) antibody

Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX82728

Cat. No. GTX82728

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Xenopus laevis
Package
100 μl ($499)
See all CDC2 products

APPLICATION

Application Note

Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM HEPES (pH7.5), 150mM NaCl, 100μg/ml BSA, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 conjugated to KLH

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin dependent kinase 1 , CDC2 , CDC28A , P34CDC2

Cellular Localization

Nucleus , Cytoplasm , Mitochondrion

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CDC2 (phospho Tyr15) antibody used in Western Blot (WB). GTX82728

GTX82728 WB Image

Western blot of human T47D cells showing phospho-specific immunolabeling of the ~34k cdc2 protein phosphorylated at Tyr 15. In lane 2, cells were treated with EGF leading to dephosphorylation of Tyr 15 using CDC2 (phospho Tyr15) antibody (GTX82728).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 499