APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml |
1-2μg/ml for 30 minutes at RT |
1-2μg/10⁶ cells |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Urea-denatured His6 Cdc20 human recombinant protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CDC20 , CDC20A , bA276H19.3 , cell division cycle 20 , p55CDC
Cellular Localization
Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome,Spindle pole
Background
CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX34578 IHC-P Image
IHC-P analysis of human gastric carcinoma tissue using GTX34578 CDC20 antibody [AR12].
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GTX34578 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX34578 CDC20 antibody [AR12].
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REFERENCE
There are currently no references for CDC20 antibody [AR12] (GTX34578). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CDC20 antibody [AR12] (GTX34578). Be the first to share your experience with this product.