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CDC20 antibody

Anti-CDC20 antibody used in Western Blot (WB). GTX111137
Anti-CDC20 antibody used in Western Blot (WB). GTX111137
Anti-CDC20 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX111137

Cat. No. GTX111137

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CDC20 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDC20. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036529

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 20 , CDC20A , bA276H19.3 , p55CDC

Background

CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDC20 antibody used in Western Blot (WB). GTX111137

GTX111137 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX111137 diluted at 1:1000

Anti-CDC20 antibody used in Western Blot (WB). GTX111137

GTX111137 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC20 antibody (GTX111137) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDC20 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX111137

GTX111137 WB IP Image

The data was published in the journal Nat Commun in 2016. PMID: 26743940

REFERENCE

Application Reference

Kulkarni P et al. Mol Cancer Res. 2018; 16 (11) : 1750-1760 Elevated miR-182-5p Associates with Renal Cancer Cell Mitotic Arrest through Diminished MALAT-1 Expression.
Application : WB
Reactivity : Human
Zhou H et al. Nat Commun 2016; 7 : 10151 Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169