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CDC25A antibody

Orthogonal
Anti-CDC25A antibody used in IHC (Paraffin sections) (IHC-P). GTX102308
Anti-CDC25A antibody used in Western Blot (WB). GTX102308
Anti-CDC25A antibody used in IHC (Paraffin sections) (IHC-P). GTX102308
Anti-CDC25A antibody used in Western Blot (WB). GTX102308

Cat. No. GTX102308

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CDC25A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

H1299 , MCF-7

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CDC25A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949941

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 25A , CDC25A2

Background

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDC25A antibody used in IHC (Paraffin sections) (IHC-P). GTX102308

GTX102308 IHC-P Image

CDC25A antibody detects CDC25A protein at cytoplasm in rat pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
CDC25A antibody (GTX102308) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CDC25A antibody used in Western Blot (WB). GTX102308

GTX102308 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: MCF-7
7.5% SDS PAGE
GTX102308 diluted at 1:1000

Anti-CDC25A antibody used in IHC (Paraffin sections) (IHC-P). GTX102308

GTX102308 IHC-P Image

CDC25A antibody detects CDC25A protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
CDC25A antibody (GTX102308) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CDC25A antibody used in Western Blot (WB). GTX102308

GTX102308 WB Image

Orthogonal

The data was published in the journal PLoS One in 2015.PMID: 25928539

REFERENCE

Application Reference

Wen HC et al. PLoS One 2015; 10 (4) : e0125518 Elevation of soluble guanylate cyclase suppresses proliferation and survival of human breast cancer cells.
Application : WB
Reactivity : Human
Liu LY et al. Chem Res Toxicol 2013; 26 (11) : 1683-91 N-Hydroxycinnamide derivatives of osthole presenting genotoxicity and cytotoxicity against human colon adenocarcinoma cells in vitro and in vivo.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

CDC25A antibody Cat. No. GTX102308

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-CDC25A antibody used in Western Blot (WB). GTX102308
Date : Anonymous submitted on 07-Nov-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 15μg
Blocking : 5% milk , 25°C, 1.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169