GeneTex
United States (US)

CDC25A antibody

GTX102308_39862_20160323_IHC-P_R_19011506_977.jpg
GTX102308_39862_WB_19011506_744.jpg
GTX102308_39862_20160323_IHC-P_M_19011506_110.jpg

Cat No. GTX102308

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

H1299 , MCF-7

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CDC25A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CDC25A2 antibody, CDC25A antibody, cell division cycle 25A antibody, dual specificity phosphatase CDC25A antibody, CDC25A2-CAG isoform antibody, M-phase inducer phosphatase 1 antibody, cell division cycle 25 homolog A (S. pombe) antibody

Background

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102308_39862_20160323_IHC-P_R_19011506_977.jpg

GTX102308 IHC-P Image

CDC25A antibody detects CDC25A protein at cytoplasm in rat pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
CDC25A antibody (GTX102308) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102308_39862_WB_19011506_744.jpg

GTX102308 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: MCF-7
7.5% SDS PAGE
GTX102308 diluted at 1:1000

GTX102308_39862_20160323_IHC-P_M_19011506_110.jpg

GTX102308 IHC-P Image

CDC25A antibody detects CDC25A protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
CDC25A antibody (GTX102308) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW

CDC25A antibody Cat No. GTX102308

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 07-Nov-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 15μg
Blocking : 5% milk , 25°C, 1.5Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169