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CDC25A (phospho Ser178) antibody

Anti-CDC25A (phospho Ser178) antibody used in Western Blot (WB). GTX86031
Anti-CDC25A (phospho Ser178) antibody used in IHC (Paraffin sections) (IHC-P). GTX86031

Cat. No. GTX86031

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($409)
See all CDC25A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human CDC25A (144-193).

Purification

Purified by antigen-affinity chromatography. Non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CDC25A , CDC25A2 , cell division cycle 25A

Background

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CDC25A (phospho Ser178) antibody used in Western Blot (WB). GTX86031

GTX86031 WB Image

WB analysis of A549 cells treated with UV (15mins) lysate using GTX86031 CDC25A (phospho Ser178) antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CDC25A (phospho Ser178) antibody used in IHC (Paraffin sections) (IHC-P). GTX86031

GTX86031 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX86031 CDC25A (phospho Ser178) antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409