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CDC25C antibody

KO/KD Orthogonal
Anti-CDC25C antibody used in Western Blot (WB). GTX102809
Anti-CDC25C antibody used in Western Blot (WB). GTX102809
Anti-CDC25C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102809
Anti-CDC25C antibody used in IHC (Paraffin sections) (IHC-P). GTX102809
Anti-CDC25C antibody used in Western Blot (WB). GTX102809
Anti-CDC25C antibody used in Western Blot (WB). GTX102809

Cat. No. GTX102809

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CDC25C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

A431 , HeLa

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDC25C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721969

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 25C , CDC25 , PPP1R60

Cellular Localization

Nucleus

Background

This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDC25C antibody used in Western Blot (WB). GTX102809

GTX102809 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C antibody (GTX102809) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CDC25C antibody used in Western Blot (WB). GTX102809

GTX102809 WB Image

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
GTX102809 diluted at 1:2000

Anti-CDC25C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102809

GTX102809 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using CDC25C(GTX102809) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-CDC25C antibody used in IHC (Paraffin sections) (IHC-P). GTX102809

GTX102809 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using CDC25C(GTX102809) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CDC25C antibody used in Western Blot (WB). GTX102809

GTX102809 WB Image

Orthogonal

The data was published in the journal Cell Death Dis in 2014.PMID: 25429617

Anti-CDC25C antibody used in Western Blot (WB). GTX102809

GTX102809 WB Image

Orthogonal

The data was published in the journal BMC Genomics in 2015. PMID: 25652794

REFERENCE

Application Reference

Kuo TT et al. ACS Omega 2020; 5 (49) : 31792-31800 Melissa officinalis Extract Induces Apoptosis and Inhibits Migration in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Ko PH et al. Sci Rep 2019; 9 (1) : 2147 Macrophage Migration Inhibitory Factor Acts as the Potential Target of a Newly Synthesized Compound, 1-(9'-methyl-3'-carbazole)-3, 4-dihydro-β-carboline.
Application : WB
Reactivity : Human
Lee MJ et al. Molecules 2018; 23 (10) Differential Pharmacological Activities of Oxygen Numbers on the Sulfoxide Moiety of Wasabi Compound 6-(Methylsulfinyl) Hexyl Isothiocyanate in Human Oral Cancer Cells.
Application : WB
Reactivity : Human
Lin LL et al. BMC Genomics 2015; 16 : 41 Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells.
Application : WB
Reactivity : Human
Chang HY et al. J Proteome Res 2015; 14 (2) : 1250-62 Quantitative proteomics reveals middle infrared radiation-interfered networks in breast cancer cells.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Kuo TT et al. ACS Omega 2020; 5 (49):31792-31800 Melissa officinalis Extract Induces Apoptosis and Inhibits Migration in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Ko PH et al. Sci Rep 2019; 9 (1):2147 Macrophage Migration Inhibitory Factor Acts as the Potential Target of a Newly Synthesized Compound, 1-(9'-methyl-3'-carbazole)-3, 4-dihydro-β-carboline.
Application : WB
Reactivity : Human
Lee MJ et al. Molecules 2018; 23 (10) Differential Pharmacological Activities of Oxygen Numbers on the Sulfoxide Moiety of Wasabi Compound 6-(Methylsulfinyl) Hexyl Isothiocyanate in Human Oral Cancer Cells.
Application : WB
Reactivity : Human
Lin LL et al. BMC Genomics 2015; 16:41 Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells.
Application : WB
Reactivity : Human
Chang HY et al. J Proteome Res 2015; 14 (2):1250-62 Quantitative proteomics reveals middle infrared radiation-interfered networks in breast cancer cells.
Application : WB
Reactivity : Human
Chang HY et al. Cell Death Dis 2014; 5:e1540 Combination therapy targeting ectopic ATP synthase and 26S proteasome induces ER stress in breast cancer cells.
Application : WB
Reactivity : Human
Liu LY et al. Chem Res Toxicol 2013; 26 (11):1683-91 N-Hydroxycinnamide derivatives of osthole presenting genotoxicity and cytotoxicity against human colon adenocarcinoma cells in vitro and in vivo.
Application : WB
Reactivity : Human
Chang HY et al. PLoS One 2013; 8 (1):e54117 Middle infrared radiation induces G2/M cell cycle arrest in A549 lung cancer cells.
Application : WB
Reactivity : Human
Cheung AK et al. Mol Cancer Ther 2013; 12 (8):1393-401 Polo-like kinase inhibitor Ro5203280 has potent antitumor activity in nasopharyngeal carcinoma.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169