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CDC25C antibody

Anti-CDC25C antibody used in Western Blot (WB). GTX128170
Anti-CDC25C antibody used in Western Blot (WB). GTX128170
Anti-CDC25C antibody used in Western Blot (WB). GTX128170
Anti-CDC25C antibody used in Western Blot (WB). GTX128170
Anti-CDC25C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128170

Cat No. GTX128170

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , NCI-H929 , HeLa (untreated) , HeLa (100ng/ml Nocodazole treatment for 24hr) , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CDC25C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cell division cycle 25C , CDC25 , PPP1R60

Cellular Localization

Nucleus

Background

This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDC25C antibody used in Western Blot (WB). GTX128170

GTX128170 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C antibody (GTX128170) diluted at 1:500.

Anti-CDC25C antibody used in Western Blot (WB). GTX128170

GTX128170 WB Image

CDC25C antibody detects CDC25C protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg K562 whole cell lysate/extract
D. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
CDC25C antibody (GTX128170) dilution: 1:1000

Anti-CDC25C antibody used in Western Blot (WB). GTX128170

GTX128170 WB Image

CDC25C antibody detects CDC25C protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
10 % SDS-PAGE
CDC25C antibody (GTX128170) dilution: 1:1000

Anti-CDC25C antibody used in Western Blot (WB). GTX128170

GTX128170 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C antibody (GTX128170) diluted at 1:500.

Anti-CDC25C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128170

GTX128170 ICC/IF Image

CDC25C antibody detects CDC25C protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDC25C protein stained by CDC25C antibody (GTX128170) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

REFERENCE

There are currently no references for CDC25C antibody (GTX128170). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169