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CDC25C (phospho Ser216) antibody

Anti-CDC25C (phospho Ser216) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50689
Anti-CDC25C (phospho Ser216) antibody used in Western Blot (WB). GTX50689
Anti-CDC25C (phospho Ser216) antibody used in IHC (Paraffin sections) (IHC-P). GTX50689

Cat. No. GTX50689

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all CDC25C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 216 (S-P-S(p)-M-P) derived from human CDC25C.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 25C , CDC25 , PPP1R60

Cellular Localization

Nucleus

Background

This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-CDC25C (phospho Ser216) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50689

GTX50689 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50689 CDC25C (phospho Ser216) antibody.

Anti-CDC25C (phospho Ser216) antibody used in Western Blot (WB). GTX50689

GTX50689 WB Image

WB analysis of extracts from 293 cells untreated or treated with serum using GTX50689 CDC25C (phospho Ser216) antibody.

Anti-CDC25C (phospho Ser216) antibody used in IHC (Paraffin sections) (IHC-P). GTX50689

GTX50689 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50689 CDC25C (phospho Ser216) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399