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CDC25C (phospho Thr48) antibody

Orthogonal
Anti-CDC25C (phospho Thr48) antibody used in Western Blot (WB). GTX17679
Anti-CDC25C (phospho Thr48) antibody used in IHC (Paraffin sections) (IHC-P). GTX17679
Anti-CDC25C (phospho Thr48) antibody used in ELISA (ELISA). GTX17679

Cat. No. GTX17679

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($419)
See all CDC25C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P Assay dependent
ELISA 1:20000-1:40000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human cdc25C around the phosphorylation site of Thr48.

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cell division cycle 25C , CDC25 , PPP1R60

Cellular Localization

Nucleus

Background

This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-CDC25C (phospho Thr48) antibody used in Western Blot (WB). GTX17679

GTX17679 WB Image

Orthogonal

WB analysis of RAW264.7 lysates with P (phospho-) and N (non phospho-) peptide using GTX17679 CDC25C (phospho Thr48) antibody.

Anti-CDC25C (phospho Thr48) antibody used in IHC (Paraffin sections) (IHC-P). GTX17679

GTX17679 IHC-P Image

IHC-P analysis of human gastric cancer tissue sections using GTX17679 CDC25C (phospho Thr48) antibody.
Antigen retireval : Heat mediated antigen retrieval in citrate buffer
Dilution : 1:100

Anti-CDC25C (phospho Thr48) antibody used in ELISA (ELISA). GTX17679

GTX17679 ELISA Image

ELISA analysis of P (phospho-) and N (non phosho-) immunogen peptide using GTX17679 CDC25C (phospho Thr48) antibody.
Peptides concentration: 1μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419