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CDC27 antibody [5C12]

Anti-CDC27 antibody [5C12] used in IHC (Paraffin sections) (IHC-P). GTX82818
Anti-CDC27 antibody [5C12] used in Western Blot (WB). GTX82818

Cat. No. GTX82818

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5C12

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all CDC27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CDC27 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 27 , ANAPC3 , APC3 , CDC27Hs , D0S1430E , D17S978E , H-NUC , HNUC , NUC2

Cellular Localization

Nucleus

Background

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-CDC27 antibody [5C12] used in IHC (Paraffin sections) (IHC-P). GTX82818

GTX82818 IHC-P Image

IHC-P analysis of lung cancer tissue (left) and colon cancer tissue (right) using GTX82818 CDC27 antibody [5C12].

Anti-CDC27 antibody [5C12] used in Western Blot (WB). GTX82818

GTX82818 WB Image

WB analysis of CDC27(AA: 724-830)-hIgGFc transfected HEK293 cell using GTX82818 CDC27 antibody [5C12].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399