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CDC27 antibody [AF3.1]

Cat. No. GTX10538

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AF3.1

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IP, ELISA

Reactivity

 Human, Mouse, Rat, Bovine, Dog, Xenopus
Package
100 μl ($399)
See all CDC27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

HeLa nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~2? mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic peptide corresponding to the C-terminus 10 residues of human Cdc27.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

RRID

AB_367646

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 27 , ANAPC3 , APC3 , CDC27Hs , D0S1430E , D17S978E , H-NUC , HNUC , NUC2

Cellular Localization

Nucleus

Background

Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.

Database

Research Area

REFERENCE

Application Reference

Senese S et al. Mol Biol Cell 2015; 26 (3) : 440-52 A unique insertion in STARD9's motor domain regulates its stability.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399