GeneTex
United States (US)

CDC27 antibody

GTX17058_742_IHC-P_20180221_18121410_419.jpg
GTX17058_2346_WB_20180221_18121410_278.jpg

Cat No. GTX17058

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

Mouse Liver Tissue Lysate(GTX27935)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CDC27 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human CDC27.The immunogen is located within the last 50 amino acids of CDC27.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cell Division Cycle 27,Anapc3,Apc3,Cdc27Hs,D0S1430E,D17S978E,H-Nuc,Hnuc,Nuc2,Cdc27

Background

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX17058_742_IHC-P_20180221_18121410_419.jpg

GTX17058 IHC-P Image

IHC-P analysis of rat liver tissue using GTX17058 CDC27 antibody.
Working concentration : 5 μg/ml

GTX17058_2346_WB_20180221_18121410_278.jpg

GTX17058 WB Image

WB analysis of mouse liver tissue lysate using GTX17058 CDC27 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Package List Price ($)
$ 329