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CDC27 antibody

Anti-CDC27 antibody used in Western Blot (WB). GTX41386

Cat No. GTX41386

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
50 μg ($309)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF Assay dependent
ELISA 1:5000 - 1:40000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

Hela whole cell lysate

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: NINDSLEIT , corresponding to Internal sequence amino acids 422-430 of Human Cdc27.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cell division cycle 27 , ANAPC3 , APC3 , CDC27Hs , D0S1430E , D17S978E , H-NUC , HNUC , NUC2

Cellular Localization

Nucleus

Background

Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.

Database

Research Area

DATA IMAGES

Anti-CDC27 antibody used in Western Blot (WB). GTX41386

GTX41386 WB Image

Western blot using Affinity Purified anti-cdc27 antibody (GTX41386) shows detection of a band ~90 kDa corresponding to human cdc27 (arrowhead). Approximately 35 ug of HeLa whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 309