APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500 - 1:2000 |
| Assay dependent |
| 1:5000 - 1:40000 |
Not tested in other applications.
Calculated MW
Positive Control
Hela whole cell lysate
PROPERTIES
Form
Liquid
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide: NINDSLEIT , corresponding to Internal sequence amino acids 422-430 of Human Cdc27.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
cell division cycle 27 , ANAPC3 , APC3 , CDC27Hs , D0S1430E , D17S978E , H-NUC , HNUC , NUC2
Cellular Localization
Nucleus
Background
Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Database
Research Area
DATA IMAGES
|
GTX41386 WB Image
Western blot using Affinity Purified anti-cdc27 antibody (GTX41386) shows detection of a band ~90 kDa corresponding to human cdc27 (arrowhead). Approximately 35 ug of HeLa whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
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REFERENCE
There are currently no references for CDC27 antibody (GTX41386). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CDC27 antibody (GTX41386). Be the first to share your experience with this product.
![Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624](/upload/website/prouct_img/small/GTX108624/GTX108624_43754_20191108_WB_KO_watermark_20092220_924.jpg "Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624")
