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CDC34 antibody [90.12]

Anti-CDC34 antibody [90.12] used in Western Blot (WB). GTX70127

Cat. No. GTX70127

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 90.12

Isotype

 IgG1

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399)
See all CDC34 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 5 μg/ml
IP 1 - 5 μg/ml
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

293 cells and recombinant fusion protein

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length human Cdc34 expressed in E. coli.

Purification

Protein G affinity purified.
From hybridoma culture supernatant.

Conjugation

Unconjugated

RRID

AB_377584

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 34 , E2-CDC34 , UBC3 , UBCH3 , UBE2R1

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CDC34 antibody [90.12] used in Western Blot (WB). GTX70127

GTX70127 WB Image

Detection of human CDC34 protein in DU145 whole cell lysate using GeneTex CDC34 90.12 monoclonal antibody GTX70127.

REFERENCE

Application Reference

Fernandez-Sanchez ME et al. J Biol Chem 2010; 285 (23) : 17390-7 The human COP9 signalosome protects ubiquitin-conjugating enzyme 3 (UBC3/Cdc34) from beta-transducin repeat-containing protein (betaTrCP)-mediated degradation.
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REVIEW

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Package List Price ($)
$ 399
Custom Conjugations