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CDC34 antibody [90.12]

Anti-CDC34 antibody [90.12] used in Western Blot (WB). GTX70127

Cat No. GTX70127

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 90.12

Isotype

 IgG1

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($329)
See all CDC34 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 5 μg/ml
IP 1 - 5 μg/ml
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

293 cells and recombinant fusion protein

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, no preservative added.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length human Cdc34 expressed in E. coli.

Purification

Protein G affinity purified.
From hybridoma culture supernatant.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cell Division Cycle 34 , E2-Cdc34 , Ubc3 , Ubch3 , Ube2R1 , Cdc34

Background

Cdc34 is a ubiquitin conjugating enzyme (UBC) or E2 that has been shown to be essential for the G1 to S phase transition in S. cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In egg extracts of the frog Xenopus laevis, Cdc34 has been shown to be required for the onset of DNA replication and appears to function in a multi-protein complex. Also, Cdc34 is highly conserved among vertebrates and is likely to function in a similar manner in all eukaryotes to regulate the start of DNA synthesis.

Database

Research Area

DATA IMAGES

Anti-CDC34 antibody [90.12] used in Western Blot (WB). GTX70127

GTX70127 WB Image

Detection of human CDC34 protein in DU145 whole cell lysate using GeneTex CDC34 90.12 monoclonal antibody GTX70127.

REFERENCE

Application Reference

Fernandez-Sanchez ME et al. J Biol Chem 2010; 285 (23) : 17390-7 The human COP9 signalosome protects ubiquitin-conjugating enzyme 3 (UBC3/Cdc34) from beta-transducin repeat-containing protein (betaTrCP)-mediated degradation.
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REVIEW

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Package List Price ($)
$ 329