GeneTex
United States (US)

CDC34 antibody [N1C3]

GTX105013_39771_20150410_IFA_19011506_221.jpg
GTX105013_IFA_19011506_184.jpg
GTX105013_WB_19011506_849.jpg

Cat No. GTX105013

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Rat, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDC34. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

E2-CDC34 antibody, UBC3 antibody, UBCH3 antibody, UBE2R1 antibody, CDC34 antibody, ubiquitin-conjugating enzyme E2-32 KDA complementing antibody, ubiquitin carrier protein antibody, ubiquitin-conjugating enzyme E2 R1 antibody, ubiquitin-protein ligase R1 antibody, ubiquitin-conjugating enzyme E2-CDC34 antibody, cell division cycle 34 homolog (S. cerevisiae) antibody

Background

The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105013_39771_20150410_IFA_19011506_221.jpg

GTX105013 ICC/IF Image

CDC34 antibody [N1C3] detects CDC34 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDC34 protein stained by CDC34 antibody [N1C3] (GTX105013) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX105013_IFA_19011506_184.jpg

GTX105013 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using CDC34(GTX105013) antibody at 1:200 dilution.

GTX105013_WB_19011506_849.jpg

GTX105013 WB Image

Sample (30μg whole cell lysate)
A:Raji (GTX27908)
12% SDS PAGE
GTX105013 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169