GeneTex
  • Country / Location Selection

United States (US)

CDC34 antibody [N1C3]

Anti-CDC34 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105013
Anti-CDC34 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105013
Anti-CDC34 antibody [N1C3] used in Western Blot (WB). GTX105013

Cat No. GTX105013

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Rat, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDC34. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cell division cycle 34 , E2-CDC34 , UBC3 , UBCH3 , UBE2R1

Background

The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-CDC34 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105013

GTX105013 ICC/IF Image

CDC34 antibody [N1C3] detects CDC34 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDC34 protein stained by CDC34 antibody [N1C3] (GTX105013) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Anti-CDC34 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105013

GTX105013 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using CDC34(GTX105013) antibody at 1:200 dilution.

Anti-CDC34 antibody [N1C3] used in Western Blot (WB). GTX105013

GTX105013 WB Image

Sample (30μg whole cell lysate)
A:Raji (GTX27908)
12% SDS PAGE
GTX105013 diluted at 1:1000

REFERENCE
REVIEW
There are currently no reviews for CDC34 antibody [N1C3] (GTX105013). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169