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CDC34 antibody

Anti-CDC34 antibody used in Western Blot (WB). GTX70135

Cat. No. GTX70135

Host

Mouse

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($399)

APPLICATION

Application Note

Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

27 kDa. ( Note )

Positive Control

293

PROPERTIES

Form

Liquid

Buffer

Unpurified Serum

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Full-length human Cdc34 expressed in E. coli.

Purification

Unpurified
From polyclonal serum

Conjugation

Unconjugated

RRID

AB_377586

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 34 , E2-CDC34 , UBC3 , UBCH3 , UBE2R1

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CDC34 antibody used in Western Blot (WB). GTX70135

GTX70135 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDC34 antibody (GTX70135) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399