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CDC37 (phospho Ser13) antibody [GT1338]

Recombinant antibody Orthogonal
Anti-CDC37 (phospho Ser13) antibody [GT1338] used in Western Blot (WB). GTX03250
Anti-CDC37 (phospho Ser13) antibody [GT1338] used in Western Blot (WB). GTX03250
Anti-CDC37 (phospho Ser13) antibody [GT1338] used in Western Blot (WB). GTX03250

Cat. No. GTX03250

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1338

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all CDC37 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S13 of human CDC37.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 37 , P50CDC37

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CDC37 (phospho Ser13) antibody [GT1338] used in Western Blot (WB). GTX03250

GTX03250 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC37 (phospho Ser13) antibody [GT1338] (GTX03250) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDC37 (phospho Ser13) antibody [GT1338] used in Western Blot (WB). GTX03250

GTX03250 WB Image

Orthogonal

WB analysis of NIH-3T3 cells were treated by CIP(20uL/400ul) at 37ºC for 1 hour using GTX03250 CDC37 (phospho Ser13) antibody [GT1338].
Dilution : 1:1000
Loading : 25μg per lane

Anti-CDC37 (phospho Ser13) antibody [GT1338] used in Western Blot (WB). GTX03250

GTX03250 WB Image

Orthogonal

WB analysis of C6 cells were treated by CIP(20uL/400ul) at 37ºC for 1 hour using GTX03250 CDC37 (phospho Ser13) antibody [GT1338].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 419