Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
Reacts with the N terminus of the 69 kDa isoform of the hCdc4 protein.
PBS containing 0.02% sodium azide.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Affinity Purified IgG
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
F-Box And Wd Repeat Domain Containing 7 , Ago , Cdc4 , Fbw6 , Fbw7 , Fbx30 , Fbxo30 , Fbxw6 , Sel-10 , Sel10 , Hago , Hcdc4 , Fbxw7
Cyclin E, one of the activators of the cyclin-dependent kinase Cdk2, is critical for the initiation of DNA replication and other S-phase functions. The proper timing and amplitude of cyclin E expression seem to be important, because elevated levels of cyclin E have been associated with a variety of malignancies and constitutive expression of cyclin E leads to genomic instability. Phosphorylated cyclin E depends on the novel human gene, hCdc4, that was found to be mutated in a cell line derived from breast cancer that expressed extremely high levels of cyclin E.