APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.5 μg/mL |
| 0.5-1 μg/mL |
| 1-3 μg/10⁶ cells |
| 0.1-0.5 μg/mL |
|
Note :
IHC-P
By heat
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄
Preservative
0.05 mg sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
E.coli-derived human CDC42 recombinant protein (Position: K16-C188).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cell division cycle 42 , CDC42Hs , G25K , TKS
Cellular Localization
Cell membrane , Cytoplasm, cytoskeleton, microtubule organizing center, centrosome , Midbody
Background
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]
Database
Research Area
DATA IMAGES
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GTX04584 IHC-P Image
IHC-P analysis of human colon cancer tissue using GTX04584 CDC42 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 1 μg/mL
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GTX04584 FACS Image
FACS analysis of SiHa cells using GTX04584 CDC42 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Dilution : 1 μg/10⁶ cells
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GTX04584 FACS Image
FACS analysis of U2OS cells using GTX04584 CDC42 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Dilution : 1 μg/10⁶ cells
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GTX04584 IHC-P Image
IHC-P analysis of rat intestine tissue using GTX04584 CDC42 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 1 μg/mL
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GTX04584 WB Image
WB analysis of various samples using GTX04584 CDC42 antibody.
Loading : 30 μg
Dilution : 0.5 μg/mL
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GTX04584 IHC-P Image
IHC-P analysis of human mammary cancer tissue using GTX04584 CDC42 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 1 μg/mL
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GTX04584 IHC-P Image
IHC-P analysis of mouse intestine tissue using GTX04584 CDC42 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 1 μg/mL
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REFERENCE
There are currently no references for CDC42 antibody (GTX04584). Be the first to share your publications with this product.
REVIEW
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