GeneTex
United States (US)

CDC42 antibody

GTX100904_39471_20160302_IHC-P_M_19011506_272.jpg
GTX100904_39471_WB_M_Brain_19011506_291.jpg
GTX100904_39471_20180928_WB_B_19011506_549.jpg
GTX100904_39471_20151112_WB_R_19011506_119.jpg
GTX100904_39471_20160302_IHC-P_M_2_19011506_343.jpg

Cat No. GTX100904

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Mouse brain , PC-12 , Rat2

Predict Reactivity

Zebrafish, Sheep, Drosophila, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDC42. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cell Division Cycle 42,Cdc42Hs,G25K,Tks,Cdc42

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100904_39471_20160302_IHC-P_M_19011506_272.jpg

GTX100904 IHC-P Image

CDC42 antibody detects CDC42 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CDC42 antibody (GTX100904) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100904_39471_WB_M_Brain_19011506_291.jpg

GTX100904 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX100904 diluted at 1:1000

GTX100904_39471_20180928_WB_B_19011506_549.jpg

GTX100904 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDC42 antibody (GTX100904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100904_39471_20151112_WB_R_19011506_119.jpg

GTX100904 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDC42 antibody (GTX100904) diluted at 1:500.

GTX100904_39471_20160302_IHC-P_M_2_19011506_343.jpg

GTX100904 IHC-P Image

CDC42 antibody detects CDC42 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CDC42 antibody (GTX100904) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169