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CDC42 antibody

Anti-CDC42 antibody used in Western Blot (WB). GTX54514
Anti-CDC42 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54514
Anti-CDC42 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54514
Anti-CDC42 antibody used in IHC (Paraffin sections) (IHC-P). GTX54514
Anti-CDC42 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54514
Anti-CDC42 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54514

Cat. No. GTX54514

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse, Rat, Primate
Package
100 μg ($399)
See all CDC42 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:20
IHC Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

C-Terminal truncated human recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 42 , CDC42Hs , G25K , TKS

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]

Database

Research Area

DATA IMAGES

Anti-CDC42 antibody used in Western Blot (WB). GTX54514

GTX54514 WB Image

WB analysis of 50 ug of various whole cell lysate using GTX54514 CDC42 antibody.
Dilution : 1:1000

Anti-CDC42 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54514

GTX54514 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX54514 CDC42 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-CDC42 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54514

GTX54514 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54514 CDC42 antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

Anti-CDC42 antibody used in IHC (Paraffin sections) (IHC-P). GTX54514

GTX54514 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX54514 CDC42 antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-CDC42 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54514

GTX54514 ICC/IF Image

ICC/IF analysis of A431 cells using GTX54514 CDC42 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-CDC42 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54514

GTX54514 ICC/IF Image

ICC/IF analysis of C6 cells using GTX54514 CDC42 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399