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CDC42 antibody

Anti-CDC42 antibody used in Western Blot (WB). GTX85114

Cat. No. GTX85114

Reference 1

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($419)
See all CDC42 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Human Brain Tissue Lysate(GTX27918)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CDC42 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CDC42.The immunogen is located within amino acids 40 - 90 of CDC42.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10734513

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 42 , CDC42Hs , G25K , TKS

Cellular Localization

Cell membrane

Background

CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to S. cerevisiae CDC42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. CDC42 is thought to regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. At least two isoforms of CDC42 are known to exist.

Database

Research Area

DATA IMAGES

Anti-CDC42 antibody used in Western Blot (WB). GTX85114

GTX85114 WB Image

WB analysis of human brain tissue lysate using GTX85114 CDC42 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

REFERENCE

Application Reference

Caspani EM et al. PLoS One 2014; 9 (7) : e101402 Glioblastoma: A Pathogenic Crosstalk between Tumor Cells and Pericytes.
Reactivity : Mouse
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419