CDC45L antibody [N3C3]

Name: CDC45L antibody [N3C3]
Brand: GeneTex
SKU: GTX110586
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-CDC45L antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110586

Anti-CDC45L antibody [N3C3] used in Western Blot (WB). GTX110586

Cat. No. GTX110586

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all CDC45L products

View product citations for antibody GTX110586 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

HeLa , Jurkat , Raji , NCI-H929

Product Note

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDC45L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731460

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 45 , CDC45L , CDC45L2 , MGORS7 , PORC-PI-1

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported. [provided by RefSeq]

Database

Gene ID: 8318 CDC45
UniProt: O75419 CDC45

Research Area

DATA IMAGES

GTX110586 ICC/IF Image

CDC45L antibody [N3C3] detects CDC45L protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDC45L protein stained by CDC45L antibody [N3C3] (GTX110586) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX110586 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC45L antibody [N3C3] (GTX110586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX110586 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX110586 diluted at 1:1000

REFERENCE

Application Reference

Guo L et al. Cell Death Differ 2012; 19 (12) : 1917-27 Histone demethylase Kdm4b functions as a co-factor of C/EBPβ to promote mitotic clonal expansion during differentiation of 3T3-L1 preadipocytes.

Application : WB

Reactivity : Mouse

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