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CDC6 antibody

Anti-CDC6 antibody used in Immunoprecipitation (IP). GTX00896
Anti-CDC6 antibody used in Western Blot (WB). GTX00896
Anti-CDC6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00896
Anti-CDC6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00896

Cat. No. GTX00896

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all CDC6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
ICC/IF 1:200
IP 1:200
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant GST-human CDC6 (1-326 amino acids) expressed in E. coli

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 6 , CDC18L , CDC6 , HsCDC18 , HsCDC6 , MGORS5

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CDC6 antibody used in Immunoprecipitation (IP). GTX00896

GTX00896 IP Image

IP analysis of HeLa whole cell lysate using GTX00896 CDC6 antibody.
Lane 1 : Control IP with non-immune IgG
Lane 2 : Immunoprecipitates with anti-CDC6 antibody
Lane 3 : Input

Anti-CDC6 antibody used in Western Blot (WB). GTX00896

GTX00896 WB Image

WB analysis of HeLa whole cell lysate using GTX00896 CDC6 antibody.
Dilution : 1:1000
Loading : 10 μg

Anti-CDC6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00896

GTX00896 ICC/IF Image

ICC/IF analysis of MEF cells (E14.5) using GTX00896 CDC6 antibody. Note that CDC6 abundantly localizes in nuclei of the cells at G1 and early S phase.
Fixation : 4% PFA, RT, 10 min
Permeabilization : ice-cold methanol, on ice, 10 min
Dilution : 1:200

Anti-CDC6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00896

GTX00896 ICC/IF Image

ICC/IF analysis of HeLa cells in G1 or early S phase using GTX00896 CDC6 antibody or Control non-immune antibody.
Green : control or primary antibody
Red : PI (propidium iodide)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399