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CDC6 antibody

Comparison Orthogonal
Anti-CDC6 antibody used in Western Blot (WB). GTX108979
Anti-CDC6 antibody used in Western Blot (WB). GTX108979
Anti-CDC6 antibody used in IHC (Paraffin sections) (IHC-P). GTX108979
Anti-CDC6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108979
Anti-CDC6 antibody used in Western Blot (WB). GTX108979
Anti-CDC6 antibody used in Western Blot (WB). GTX108979

Cat. No. GTX108979

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CDC6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

Neuro2A , GL261 , C8D30 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , Jurkat , HeLa (10 mM Hydroxyurea treatment for 18 hr)

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDC6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618065

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 6 , CDC18L , HsCDC18 , HsCDC6 , MGORS5

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDC6 antibody used in Western Blot (WB). GTX108979

GTX108979 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CDC6 antibody (GTX108979) diluted at 1:500 and competitor's antibody (sc-8341) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CDC6 antibody used in Western Blot (WB). GTX108979

GTX108979 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC6 antibody (GTX108979) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDC6 antibody used in IHC (Paraffin sections) (IHC-P). GTX108979

GTX108979 IHC-P Image

CDC6 antibody detects CDC6 protein at cytoplasm in human oral carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
CDC6 antibody (GTX108979) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CDC6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108979

GTX108979 ICC/IF Image

CDC6 antibody detects CDC6 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDC6 protein stained by CDC6 antibody (GTX108979) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-CDC6 antibody used in Western Blot (WB). GTX108979

GTX108979 WB Image

Cdc6 antibody detects CDC6 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
Cdc6 antibody (GTX108979) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDC6 antibody used in Western Blot (WB). GTX108979

GTX108979 WB Image

Cdc6 antibody detects CDC6 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg BCL-1 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
F. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
Cdc6 antibody (GTX108979) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

van Kempen LC et al. Sci Transl Med 2016; 8 (369) : 369ra177 The protein phosphatase 2A regulatory subunit PR70 is a gonosomal melanoma tumor suppressor gene.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169