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CDC73 antibody

Anti-CDC73 antibody used in IHC (Paraffin sections) (IHC-P). GTX04105
Anti-CDC73 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04105
Anti-CDC73 antibody used in Flow cytometry (FACS). GTX04105
Anti-CDC73 antibody used in Western Blot (WB). GTX04105
Anti-CDC73 antibody used in IHC (Paraffin sections) (IHC-P). GTX04105
Anti-CDC73 antibody used in Western Blot (WB). GTX04105

Cat. No. GTX04105

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all CDC73 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:10-1:50
IHC-P 1:25
FACS 1:25
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 132-161 amino acids from the Central region of human CDC73.

Purification

Protein A purified followed by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 73 , C1orf28 , FIHP , HPTJT , HRPT1 , HRPT2 , HYX

Cellular Localization

Nucleus

Background

This gene encodes a tumor suppressor that is involved in transcriptional and post-transcriptional control pathways. The protein is a component of the the PAF protein complex, which associates with the RNA polymerase II subunit POLR2A and with a histone methyltransferase complex. This protein appears to facilitate the association of 3' mRNA processing factors with actively-transcribed chromatin. Mutations in this gene have been linked to hyperparathyroidism-jaw tumor syndrome, familial isolated hyperparathyroidism, and parathyroid carcinoma. [provided by RefSeq, Jul 2009]

Database

Research Area

DATA IMAGES

Anti-CDC73 antibody used in IHC (Paraffin sections) (IHC-P). GTX04105

GTX04105 IHC-P Image

IHC-P analysis of human adrenal gland tissue sections using GTX04105 CDC73 antibody.
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
Dilution : 1:25

Anti-CDC73 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04105

GTX04105 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX04105 CDC73 antibody.
Green : Primary antibody
Red : actin

Anti-CDC73 antibody used in Flow cytometry (FACS). GTX04105

GTX04105 FACS Image

FACS analysis of 2% paraformaldehyde-fixed U2OS cells using GTX04105 CDC73 antibody. Acquisition of >10, 000 events was performed.
Green : Primary antibody
Blue : Isotype control
Permeabilization : 90% methanol for 10 min
Dilution : 1:25

Anti-CDC73 antibody used in Western Blot (WB). GTX04105

GTX04105 WB Image

WB analysis of 293 cell lysates using GTX04105 CDC73 antibody.
Loading : 35 μg/lane

Anti-CDC73 antibody used in IHC (Paraffin sections) (IHC-P). GTX04105

GTX04105 IHC-P Image

IHC-P analysis of human adrenal gland tissue sections using GTX04105 CDC73 antibody.
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
Dilution : 1:25

Anti-CDC73 antibody used in Western Blot (WB). GTX04105

GTX04105 WB Image

WB analysis of varoius sample lysates using GTX04105 CDC73 antibody.
Lane 1 : 293T/17 cells
Lane 2 : TT cells
Dilution : 1:2000
Loading : 20 μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 399