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CDCA4 antibody

Anti-CDCA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX87853
Anti-CDCA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87853

Cat. No. GTX87853

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all CDCA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human CDCA4 (121-170).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CDCA4 , HEPP , SEI3/HEPP , cell division cycle associated 4

Cellular Localization

Nucleus

Background

This gene encodes a protein that belongs to the E2F family of transcription factors. This protein regulates E2F-dependent transcriptional activation and cell proliferation, mainly through the E2F/retinoblastoma protein pathway. It also functions in the regulation of JUN oncogene expression. This protein shows distinctive nuclear-mitotic apparatus distribution, it is involved in spindle organization from prometaphase, and may also play a role as a midzone factor involved in chromosome segregation or cytokinesis. Two alternatively spliced transcript variants encoding the same protein have been noted for this gene. Two pseudogenes have also been identified on chromosome 1. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-CDCA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX87853

GTX87853 IHC-P Image

IHC-P analysis of human brain tissue using GTX87853 CDCA4 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CDCA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87853

GTX87853 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX87853 CDCA4 antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399