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CDH11 antibody

Anti-CDH11 antibody used in IHC (Paraffin sections) (IHC-P). GTX04544
Anti-CDH11 antibody used in Flow cytometry (FACS). GTX04544
Anti-CDH11 antibody used in IHC (Paraffin sections) (IHC-P). GTX04544
Anti-CDH11 antibody used in Western Blot (WB). GTX04544

Cat. No. GTX04544

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($469)
See all CDH11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5 μg/mL
IHC-P 2-5 μg/mL
FACS 1-3 μg/1x10⁶ cells
ELISA 0.1-0.5 μg/mL
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2 mg Na₂HPO₄

Preservative

No preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human OB Cadherin/CDH11 recombinant protein (Position: K371-S796).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 11 , CAD11 , CDHOB , ESWS , OB , OSF-4

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CDH11 antibody used in IHC (Paraffin sections) (IHC-P). GTX04544

GTX04544 IHC-P Image

IHC-P analysis of human prostate cancer tissue using GTX04544 CDH11 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH8.0)
Dilution : 2 μg/mL

Anti-CDH11 antibody used in Flow cytometry (FACS). GTX04544

GTX04544 FACS Image

FACS analysis of PC-3 cells using GTX04544 CDH11 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Dilution : 1 μg/1x10⁶ cells

Anti-CDH11 antibody used in IHC (Paraffin sections) (IHC-P). GTX04544

GTX04544 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX04544 CDH11 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH8.0)
Dilution : 2 μg/mL

Anti-CDH11 antibody used in Western Blot (WB). GTX04544

GTX04544 WB Image

WB analysis of various whole cell lysates using GTX04544 CDH11 antibody.
Lane 1: PC-3 whole cell lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Mouse brain tissue lysate
Dilution : 0.5 μg/mL
Loading : 50 μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 469