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CDH11 antibody

Anti-CDH11 antibody used in Western Blot (WB). GTX109792
Anti-CDH11 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109792
Anti-CDH11 antibody used in Western Blot (WB). GTX109792

Cat. No. GTX109792

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CDH11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CDH11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618693

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 11 , CAD11 , CDHOB , ESWS , OB , OSF-4

Cellular Localization

Cell membrane

Background

This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDH11 antibody used in Western Blot (WB). GTX109792

GTX109792 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CDH11 antibody (GTX109792) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CDH11 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109792

GTX109792 IHC-Fr Image

CDH11 antibody detects CDH11 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: CDH11 stained by CDH11 antibody (GTX109792) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-CDH11 antibody used in Western Blot (WB). GTX109792

GTX109792 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CDH11 antibody (GTX109792) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Giuseppe Pannone et al. Biomolecules 2022; 12 (4) Expression of Beta-Catenin, Cadherins and P-Runx2 in Fibro-Osseous Lesions of the Jaw: Tissue Microarray Study.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169