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CDH13 antibody [GT1255]

Recombinant antibody
Anti-CDH13 antibody [GT1255] used in Western Blot (WB). GTX02852
Anti-CDH13 antibody [GT1255] used in Western Blot (WB). GTX02852
Anti-CDH13 antibody [GT1255] used in Western Blot (WB). GTX02852
Anti-CDH13 antibody [GT1255] used in IHC (Paraffin sections) (IHC-P). GTX02852
Anti-CDH13 antibody [GT1255] used in IHC (Paraffin sections) (IHC-P). GTX02852
Anti-CDH13 antibody [GT1255] used in Western Blot (WB). GTX02852
Anti-CDH13 antibody [GT1255] used in IHC (Paraffin sections) (IHC-P). GTX02852

Cat. No. GTX02852

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1255

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all CDH13 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Observed MW

105|130 kDa. The observed M.W. is based on the following publication. PMID: 18411300|20394625

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human H Cadherin

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 13 , CDHH , P105

Background

This gene encodes a member of the cadherin superfamily. The encoded protein is localized to the surface of the cell membrane and is anchored by a GPI moiety, rather than by a transmembrane domain. The protein lacks the cytoplasmic domain characteristic of other cadherins, and so is not thought to be a cell-cell adhesion glycoprotein. This protein acts as a negative regulator of axon growth during neural differentiation. It also protects vascular endothelial cells from apoptosis due to oxidative stress, and is associated with resistance to atherosclerosis. The gene is hypermethylated in many types of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-CDH13 antibody [GT1255] used in Western Blot (WB). GTX02852

GTX02852 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CDH13 antibody [GT1255] (GTX02852) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDH13 antibody [GT1255] used in Western Blot (WB). GTX02852

GTX02852 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CDH13 antibody [GT1255] (GTX02852) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CDH13 antibody [GT1255] used in Western Blot (WB). GTX02852

GTX02852 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CDH13 antibody [GT1255] (GTX02852) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CDH13 antibody [GT1255] used in IHC (Paraffin sections) (IHC-P). GTX02852

GTX02852 IHC-P Image

IHC-P analysis of human esophageal tissue section using GTX02852 CDH13 antibody [GT1255].
Dilution : 1:100

Anti-CDH13 antibody [GT1255] used in IHC (Paraffin sections) (IHC-P). GTX02852

GTX02852 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX02852 CDH13 antibody [GT1255].
Dilution : 1:100

Anti-CDH13 antibody [GT1255] used in Western Blot (WB). GTX02852

GTX02852 WB Image

WB analysis of mouse heart tissue lysates using GTX02852 CDH13 antibody [GT1255].
Dilution : 1:1000
Loading : 25μg

Anti-CDH13 antibody [GT1255] used in IHC (Paraffin sections) (IHC-P). GTX02852

GTX02852 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX02852 CDH13 antibody [GT1255].
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 419