APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human full-length CDH16 protein
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CDH16 , cadherin 16 , Cadherin 16 , KSP-cadherin
Cellular Localization
Cell membrane
Background
This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]
Database
Research Area
DATA IMAGES
![Anti-CDH16 antibody [CDH16/1532R] used in Flow cytometry (FACS). GTX34583](/templates/default/js/lightbox/images/loading.gif "Anti-CDH16 antibody [CDH16/1532R] used in Flow cytometry (FACS). GTX34583")
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GTX34583 FACS Image
FACS analysis of HEK293 cells using GTX34583 CDH16 antibody [CDH16/1532R].
Blue : Primary antibody
Red : Isotype control
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GTX34583 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX34583 CDH16 antibody [CDH16/1532R].
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GTX34583 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX34583 CDH16 antibody [CDH16/1532R].
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GTX34583 WB Image
WB analysis of human kidney tissue lysate using GTX34583 CDH16 antibody [CDH16/1532R].
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GTX34583 IHC-P Image
IHC-P analysis of human renal cell carcinoma tissue using GTX34583 CDH16 antibody [CDH16/1532R].
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REFERENCE
There are currently no references for CDH16 antibody [CDH16/1532R] (GTX34583). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CDH16 antibody [CDH16/1532R] (GTX34583). Be the first to share your experience with this product.
![Anti-CDH16 antibody [CDH16/1532R] used in Flow cytometry (FACS). GTX34583](/upload/website/prouct_img/small/GTX34583/GTX34583_20200115_FACS_101_w_23060801_925.webp "Anti-CDH16 antibody [CDH16/1532R] used in Flow cytometry (FACS). GTX34583")
![Anti-CDH16 antibody [CDH16/1532R] used in IHC (Paraffin sections) (IHC-P). GTX34583](/upload/website/prouct_img/small/GTX34583/GTX34583_20200115_IHC-P_1623_w_23060801_641.webp "Anti-CDH16 antibody [CDH16/1532R] used in IHC (Paraffin sections) (IHC-P). GTX34583")
![Anti-CDH16 antibody [CDH16/1532R] used in IHC (Paraffin sections) (IHC-P). GTX34583](/upload/website/prouct_img/small/GTX34583/GTX34583_20200115_IHC-P_1840_w_23060801_714.webp "Anti-CDH16 antibody [CDH16/1532R] used in IHC (Paraffin sections) (IHC-P). GTX34583")
![Anti-CDH16 antibody [CDH16/1532R] used in Western Blot (WB). GTX34583](/upload/website/prouct_img/small/GTX34583/GTX34583_20200115_WB_660_w_23060801_664.webp "Anti-CDH16 antibody [CDH16/1532R] used in Western Blot (WB). GTX34583")
![Anti-CDH16 antibody [CDH16/1532R] used in IHC (Paraffin sections) (IHC-P). GTX34583](/upload/website/prouct_img/small/GTX34583/GTX34583_20200115_IHC-P_1076_w_23060801_978.webp "Anti-CDH16 antibody [CDH16/1532R] used in IHC (Paraffin sections) (IHC-P). GTX34583")
![Anti-CDH16 antibody [CDH16/1532R] used in Flow cytometry (FACS). GTX34583](/upload/website/prouct_img/normal/GTX34583/GTX34583_20200115_FACS_101_w_23060801_925.webp "Anti-CDH16 antibody [CDH16/1532R] used in Flow cytometry (FACS). GTX34583")
![Anti-CDH16 antibody [CDH16/1532R] used in IHC (Paraffin sections) (IHC-P). GTX34583](/upload/website/prouct_img/normal/GTX34583/GTX34583_20200115_IHC-P_1623_w_23060801_641.webp "Anti-CDH16 antibody [CDH16/1532R] used in IHC (Paraffin sections) (IHC-P). GTX34583")
![Anti-CDH16 antibody [CDH16/1532R] used in IHC (Paraffin sections) (IHC-P). GTX34583](/upload/website/prouct_img/normal/GTX34583/GTX34583_20200115_IHC-P_1840_w_23060801_714.webp "Anti-CDH16 antibody [CDH16/1532R] used in IHC (Paraffin sections) (IHC-P). GTX34583")
![Anti-CDH16 antibody [CDH16/1532R] used in Western Blot (WB). GTX34583](/upload/website/prouct_img/normal/GTX34583/GTX34583_20200115_WB_660_w_23060801_664.webp "Anti-CDH16 antibody [CDH16/1532R] used in Western Blot (WB). GTX34583")
![Anti-CDH16 antibody [CDH16/1532R] used in IHC (Paraffin sections) (IHC-P). GTX34583](/upload/website/prouct_img/normal/GTX34583/GTX34583_20200115_IHC-P_1076_w_23060801_978.webp "Anti-CDH16 antibody [CDH16/1532R] used in IHC (Paraffin sections) (IHC-P). GTX34583")