CDK1 / CDC2 (phospho Thr161) + CDK2 (phospho Thr160) + CDK3 (phospho Thr160) antibody [HL3648]

Specificity test was performed by coating the plate with recombinant CDK1 / CDC2 non-phospho peptide, and CDK1 / CDC2 (phospho Thr161) peptide, CDK2 non-phospho peptide, CDK2 (phospho Thr160) peptide, CDK3 non-phospho peptide, and CDK3 (phospho Thr160) peptide (15.63 ng/mL). Coated protein was probed with CDK1 / CDC2 (phospho Thr161) + CDK2 (phospho Thr160) + CDK3 (phospho Thr160) antibody [HL3648] (GTX641639) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK1 / CDC2 (phospho Thr161) + CDK2 (phospho Thr160) + CDK3 (phospho Thr160) antibody [HL3648] (GTX641639) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.