APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human CDK2 expressed in E. Coli.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
cyclin dependent kinase 2 , CDKN2 , p33(CDK2)
Cellular Localization
Cytoplasm,Endosome
Background
This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Database
Research Area
DATA IMAGES
![Anti-CDK2 antibody [1A6] used in IHC (Paraffin sections) (IHC-P). GTX60572](/templates/default/js/lightbox/images/loading.gif "Anti-CDK2 antibody [1A6] used in IHC (Paraffin sections) (IHC-P). GTX60572")
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GTX60572 IHC-P Image
IHC-P analysis of colon cancer tissue using GTX60572 CDK2 antibody [1A6].
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GTX60572 FACS Image
FACS analysis of Jurkat cells using GTX60572 CDK2 antibody [1A6].
Green : CDK2
Red : negative control
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GTX60572 ELISA Image
ELISA analysis of antigen using GTX60572 CDK2 antibody [1A6].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60572 WB Image
WB analysis of human CDK2 (AA: 197-295) recombinant protein using GTX60572 CDK2 antibody [1A6].
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GTX60572 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60572 CDK2 antibody [1A6].
Green : CDK2
Red: Actin filaments
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GTX60572 WB Image
WB analysis of Jurkat (1), HL-60 (2), K562(3), A431(4), HeLa(5), and NIH3T3 (6) cell lysate using GTX60572 CDK2 antibody [1A6].
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GTX60572 IHC-P Image
IHC-P analysis of cervical cancer tissue using GTX60572 CDK2 antibody [1A6].
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REFERENCE
There are currently no references for CDK2 antibody [1A6] (GTX60572). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CDK2 antibody [1A6] (GTX60572). Be the first to share your experience with this product.
![Anti-CDK2 antibody [1A6] used in IHC (Paraffin sections) (IHC-P). GTX60572](/upload/website/prouct_img/small/GTX60572/GTX60572_20170912_IHC-P_1_18121411_584.jpg "Anti-CDK2 antibody [1A6] used in IHC (Paraffin sections) (IHC-P). GTX60572")
![Anti-CDK2 antibody [1A6] used in Flow cytometry (FACS). GTX60572](/upload/website/prouct_img/small/GTX60572/GTX60572_20170912_FACS_18121411_781.jpg "Anti-CDK2 antibody [1A6] used in Flow cytometry (FACS). GTX60572")
![Anti-CDK2 antibody [1A6] used in ELISA (ELISA). GTX60572](/upload/website/prouct_img/small/GTX60572/GTX60572_20170912_ELISA_18121411_977.jpg "Anti-CDK2 antibody [1A6] used in ELISA (ELISA). GTX60572")
![Anti-CDK2 antibody [1A6] used in Western Blot (WB). GTX60572](/upload/website/prouct_img/small/GTX60572/GTX60572_20170912_WB_18121411_620.jpg "Anti-CDK2 antibody [1A6] used in Western Blot (WB). GTX60572")
![Anti-CDK2 antibody [1A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60572](/upload/website/prouct_img/small/GTX60572/GTX60572_20170912_ICCIF_18121411_267.jpg "Anti-CDK2 antibody [1A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60572")
![Anti-CDK2 antibody [1A6] used in Western Blot (WB). GTX60572](/upload/website/prouct_img/small/GTX60572/GTX60572_20170912_WB_1_18121411_161.jpg "Anti-CDK2 antibody [1A6] used in Western Blot (WB). GTX60572")
![Anti-CDK2 antibody [1A6] used in IHC (Paraffin sections) (IHC-P). GTX60572](/upload/website/prouct_img/small/GTX60572/GTX60572_20170912_IHC-P_18121411_903.jpg "Anti-CDK2 antibody [1A6] used in IHC (Paraffin sections) (IHC-P). GTX60572")
![Anti-CDK2 antibody [1A6] used in IHC (Paraffin sections) (IHC-P). GTX60572](/upload/website/prouct_img/normal/GTX60572/GTX60572_20170912_IHC-P_1_18121411_584.jpg "Anti-CDK2 antibody [1A6] used in IHC (Paraffin sections) (IHC-P). GTX60572")
![Anti-CDK2 antibody [1A6] used in Flow cytometry (FACS). GTX60572](/upload/website/prouct_img/normal/GTX60572/GTX60572_20170912_FACS_18121411_781.jpg "Anti-CDK2 antibody [1A6] used in Flow cytometry (FACS). GTX60572")
![Anti-CDK2 antibody [1A6] used in ELISA (ELISA). GTX60572](/upload/website/prouct_img/normal/GTX60572/GTX60572_20170912_ELISA_18121411_977.jpg "Anti-CDK2 antibody [1A6] used in ELISA (ELISA). GTX60572")
![Anti-CDK2 antibody [1A6] used in Western Blot (WB). GTX60572](/upload/website/prouct_img/normal/GTX60572/GTX60572_20170912_WB_18121411_620.jpg "Anti-CDK2 antibody [1A6] used in Western Blot (WB). GTX60572")
![Anti-CDK2 antibody [1A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60572](/upload/website/prouct_img/normal/GTX60572/GTX60572_20170912_ICCIF_18121411_267.jpg "Anti-CDK2 antibody [1A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60572")
![Anti-CDK2 antibody [1A6] used in Western Blot (WB). GTX60572](/upload/website/prouct_img/normal/GTX60572/GTX60572_20170912_WB_1_18121411_161.jpg "Anti-CDK2 antibody [1A6] used in Western Blot (WB). GTX60572")
![Anti-CDK2 antibody [1A6] used in IHC (Paraffin sections) (IHC-P). GTX60572](/upload/website/prouct_img/normal/GTX60572/GTX60572_20170912_IHC-P_18121411_903.jpg "Anti-CDK2 antibody [1A6] used in IHC (Paraffin sections) (IHC-P). GTX60572")