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CDK2 antibody [N1C3]

KO/KD Orthogonal Overexpression
Anti-CDK2 antibody [N1C3] used in Western Blot (WB). GTX101226
Anti-CDK2 antibody [N1C3] used in Western Blot (WB). GTX101226
Anti-CDK2 antibody [N1C3] used in Western Blot (WB). GTX101226

Cat. No. GTX101226

Reference 9
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Japanese Medaka
Package
100 μl ($399),
25 μl ($169)
See all CDK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

human CD2K-transfected 293T cells

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240611

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 2 , CDKN2 , p33(CDK2)

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDK2 antibody [N1C3] used in Western Blot (WB). GTX101226

GTX101226 WB Image

KO/KD

Wild-type (WT) and CDK2 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 antibody [N1C3] (GTX101226) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CDK2 antibody [N1C3] used in Western Blot (WB). GTX101226

GTX101226 WB Image

Overexpression

CDK2 antibody [N1C3] detects CDK2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human CD2K-transfected 293T cells
12% SDS-PAGE
CDK2 antibody [N1C3] (GTX101226) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDK2 antibody [N1C3] used in Western Blot (WB). GTX101226

GTX101226 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28134285

REFERENCE

Application Reference

Mao BH et al. Part Fibre Toxicol 2022; 19 (1) : 6 Use of an in silico knowledge discovery approach to determine mechanistic studies of silver nanoparticles-induced toxicity from in vitro to in vivo.
Application : WB
Reactivity : Human
Liang ML et al. Cancers (Basel) 2020; 12 (12) Abemaciclib, A Selective CDK4/6 Inhibitor, Restricts the Growth of Pediatric Ependymomas.
Application : WB
Reactivity : Human
Liu YH et al. Phytomedicine 2018; 46 : 184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
Li JM et al. Ther Adv Med Oncol 2018; 10 : 1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.
Application : WB
Reactivity : Human
Lu WJ et al. Food Funct 2018; 9 (8) : 4500-4507 Licochalcone A attenuates glioma cell growth in vitro and in vivo through cell cycle arrest.
Application : WB
Reactivity : Human
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REVIEW

CDK2 antibody [N1C3] Cat. No. GTX101226

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-CDK2 antibody [N1C3] used in Western Blot (WB). GTX101226
Date : Anonymous submitted on 04-Oct-2019
Score :
Application Tested : WB
Sample Species : Japanese Medaka
Sample : HEK293T cells transfected with pCMV-medaka CDK2
Amount used : 5 μg
Blocking : 5% skim milk in TPBS, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Lane Description : Lane 1: HEK293T transfected with mock vector; Lane 2: HEK293T transfected with pCMV-medaka CDK2
Anti-CDK2 antibody [N1C3] used in Western Blot (WB). GTX101226
Date : Anonymous submitted on 24-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 60 μg
Blocking : 5 % milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: HeLa whole cell lysate, Lane 2: HeLa-I5 whole cell lysate (HeLa-I5 isselected from HeLa by transwell invasion assay)
Submit a Review
Application Reference
Mao BH et al. Part Fibre Toxicol 2022; 19 (1):6 Use of an in silico knowledge discovery approach to determine mechanistic studies of silver nanoparticles-induced toxicity from in vitro to in vivo.
Application : WB
Reactivity : Human
Liang ML et al. Cancers (Basel) 2020; 12 (12) Abemaciclib, A Selective CDK4/6 Inhibitor, Restricts the Growth of Pediatric Ependymomas.
Application : WB
Reactivity : Human
Liu YH et al. Phytomedicine 2018; 46:184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
Li JM et al. Ther Adv Med Oncol 2018; 10:1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.
Application : WB
Reactivity : Human
Lu WJ et al. Food Funct 2018; 9 (8):4500-4507 Licochalcone A attenuates glioma cell growth in vitro and in vivo through cell cycle arrest.
Application : WB
Reactivity : Human
Yuan SY et al. Oncol Rep 2017; 37 (6):3606-3616 * induces apoptosis via the death receptor 5-dependent and mitochondrial-mediated pathways in human bladder cancer cells.
Application : WB
Reactivity : Human
Liu YH et al. Sci Rep 2017; 7:41437 Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.
Application : WB
Reactivity : Human
Kuo CY et al. Cell Cycle 2016; 15 (6):787-98 RNF4 regulates DNA double-strand break repair in a cell cycle-dependent manner.
Application : WB
Reactivity : Human
Huang AM et al. Biochem Pharmacol 2011; 82 (1):35-42 ube2m-mediated p27(kip1) degradation in * cytotoxicity.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169