GeneTex
United States (US)

CDK2 antibody [N1C3]

KO/KD Overexpression
GTX101226_41346_20180323_WB_KO_watermark_19011506_442.jpg
GTX101226_41346_WB_B_19011506_159.jpg

Cat No. GTX101226

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , human CD2K-transfected 293T

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin Dependent Kinase 2,Cdkn2,P33(Cdk2),Cdk2

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101226_41346_20180323_WB_KO_watermark_19011506_442.jpg

GTX101226 WB Image

KO/KD

Wild-type (WT) and CDK2 knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 antibody [N1C3] (GTX101226) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX101226_41346_WB_B_19011506_159.jpg

GTX101226 WB Image

Overexpression

CDK2 antibody [N1C3] detects CDK2 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human CD2K-transfected 293T cells
12% SDS-PAGE
CDK2 antibody [N1C3] (GTX101226) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

CDK2 antibody [N1C3] Cat No. GTX101226

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 24-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 60 μg
Blocking : 5 % milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Lane Description : Lane 1: HeLa whole cell lysate, Lane 2: HeLa-I5 whole cell lysate (HeLa-I5 isselected from HeLa by transwell invasion assay)
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169