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CDK2 (phospho Thr160) antibody

Anti-CDK2 (phospho Thr160) antibody used in IHC (Paraffin sections) (IHC-P). GTX79113
Anti-CDK2 (phospho Thr160) antibody used in Western Blot (WB). GTX79113

Cat. No. GTX79113

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human CDK2 around the phosphorylation site of threonine 160 (T-Y-Tp-H-E).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11173036

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CDK2,CDKN2,cyclin dependent kinase 2,p33(CDK2)

Cellular Localization

Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome,Nucleus,Cajal body,Endosome

Background

This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-CDK2 (phospho Thr160) antibody used in IHC (Paraffin sections) (IHC-P). GTX79113

GTX79113 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX79113 CDK2 (phospho Thr160) antibody.

Anti-CDK2 (phospho Thr160) antibody used in Western Blot (WB). GTX79113

GTX79113 WB Image

WB analysis of A2780 cells (Lane 1 and 2) and MDA-MB-435 cells (Lane 3) lysates using GTX79113 CDK2 (phospho Thr160) antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399