APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Based on internal testing, this antibody may cross react with CDK1 (CDC2), CDK3, and CDK5 proteins when phosphorylated at tyrosine 15.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide corresponding to residues around human CDK2 (phospho Tyr15). The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cyclin dependent kinase 2 , CDKN2 , p33(CDK2)
Cellular Localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Cytoplasm,Endosome
Background
This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Database
Research Area
DATA IMAGES
![Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948](/templates/default/js/lightbox/images/loading.gif "Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948")
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GTX636948 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636948 Image
Specificity test was performed by coating the plate with CDK1/2/3/5 peptide and CDK1/2/3/5 (phospho Tyr15) peptide (15.63 ng/mL). Coated peptides were probed with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
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GTX636948 WB Image
Drosophila tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636948 WB Image
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636948 WB Image
Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636948 IHC-P Image
CDK2 (phospho Tyr15) antibody [HL1474] detects CDK2 (phospho Tyr15) protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.CDK2 (phospho Tyr15) stained by CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948). Be the first to share your experience with this product.
![Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948](/upload/website/prouct_img/small/GTX636948/GTX636948_T-44662_20230714_WB_treatment_CIP_23071822_239.webp "Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948")
![Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in (). GTX636948](/upload/website/prouct_img/small/GTX636948/GTX636948_44711_20230721_ELISA_Indirect_Multiple_Bar_23072519_460.webp "Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in (). GTX636948")
![Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948](/upload/website/prouct_img/small/GTX636948/GTX636948_44711_20230721_WB_Drosophila_brain_23073119_414.webp "Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948")
![Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948](/upload/website/prouct_img/small/GTX636948/GTX636948_T-44662_20230714_WB_M_treatment_CIP_23071822_229.webp "Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948")
![Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948](/upload/website/prouct_img/small/GTX636948/GTX636948_T-44662_20230714_WB_R_treatment_CIP_23071822_308.webp "Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948")
![Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in IHC (Paraffin sections) (IHC-P). GTX636948](/upload/website/prouct_img/small/GTX636948/GTX636948_44711_20240401_IHC-P_M_24041019_395.webp "Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in IHC (Paraffin sections) (IHC-P). GTX636948")
![Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948](/upload/website/prouct_img/normal/GTX636948/GTX636948_T-44662_20230714_WB_treatment_CIP_23071822_239.webp "Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948")
![Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in (). GTX636948](/upload/website/prouct_img/normal/GTX636948/GTX636948_44711_20230721_ELISA_Indirect_Multiple_Bar_23072519_460.webp "Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in (). GTX636948")
![Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948](/upload/website/prouct_img/normal/GTX636948/GTX636948_44711_20230721_WB_Drosophila_brain_23073119_414.webp "Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948")
![Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948](/upload/website/prouct_img/normal/GTX636948/GTX636948_T-44662_20230714_WB_M_treatment_CIP_23071822_229.webp "Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948")
![Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948](/upload/website/prouct_img/normal/GTX636948/GTX636948_T-44662_20230714_WB_R_treatment_CIP_23071822_308.webp "Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948")
![Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in IHC (Paraffin sections) (IHC-P). GTX636948](/upload/website/prouct_img/normal/GTX636948/GTX636948_44711_20240401_IHC-P_M_24041019_395.webp "Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in IHC (Paraffin sections) (IHC-P). GTX636948")