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CDK2 (phospho Tyr15) antibody [HL1474]

Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948
Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948
Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948
Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in  (). GTX636948
Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in IHC (Paraffin sections) (IHC-P). GTX636948
Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948

Cat. No. GTX636948

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1474

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat, Drosophila
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Product Note

Based on internal testing, this antibody may cross react with CDK1 (CDC2), CDK3, and CDK5 proteins when phosphorylated at tyrosine 15.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human CDK2 (phospho Tyr15). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 2 , CDKN2 , p33(CDK2)

Cellular Localization

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Cytoplasm,Endosome

Background

This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948

GTX636948 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948

GTX636948 WB Image

Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948

GTX636948 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in  (). GTX636948

GTX636948 Image

Specificity test was performed by coating the plate with CDK1/2/3/5 peptide and CDK1/2/3/5 (phospho Tyr15) peptide (15.63 ng/mL). Coated peptides were probed with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in IHC (Paraffin sections) (IHC-P). GTX636948

GTX636948 IHC-P Image

CDK2 (phospho Tyr15) antibody [HL1474] detects CDK2 (phospho Tyr15) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
CDK2 (phospho Tyr15) stained by CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CDK2 (phospho Tyr15) antibody [HL1474] used in Western Blot (WB). GTX636948

GTX636948 WB Image

Drosophila tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

There are currently no references for CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CDK2 (phospho Tyr15) antibody [HL1474] (GTX636948). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations