Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL3327] (GTX641092) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Specificity test was performed by coating the plate with CDK1/2/3/5 peptide and CDK1/2/3/5 (phospho Tyr15) peptide (15.63 ng/mL). Coated peptides were probed with CDK2 (phospho Tyr15) antibody [HL3327] (GTX641092) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL3327] (GTX641092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Tyr15) antibody [HL3327] (GTX641092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.