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CDK4 antibody [GT624]

KO/KD
Anti-CDK4 antibody [GT624] used in Western Blot (WB). GTX629975
Anti-CDK4 antibody [GT624] used in Western Blot (WB). GTX629975

Cat. No. GTX629975

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT624

Isotype

 IgG2b

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CDK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDK4. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888182

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 4 , CMM3 , PSK-J3

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDK4 antibody [GT624] used in Western Blot (WB). GTX629975

GTX629975 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody [GT624] (GTX629975) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CDK4 antibody [GT624] used in Western Blot (WB). GTX629975

GTX629975 WB Image

CDK4 antibody [GT624] detects CDK4 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
12 % SDS-PAGE
CDK4 antibody [GT624] (GTX629975) dilution: 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations