APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CDK4. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cyclin dependent kinase 4 , CMM3 , PSK-J3
Cellular Localization
Cytoplasm,Nucleus,Membrane
Background
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-CDK4 antibody [HL1407] used in Western Blot (WB). GTX636873](/templates/default/js/lightbox/images/loading.gif "Anti-CDK4 antibody [HL1407] used in Western Blot (WB). GTX636873")
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GTX636873 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody [HL1407] (GTX636873) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636873 IHC-P Image
CDK4 antibody [HL1407] detects CDK4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
CDK4 stained by CDK4 antibody [HL1407] (GTX636873) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636873 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody [HL1407] (GTX636873) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636873 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody [HL1407] (GTX636873) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for CDK4 antibody [HL1407] (GTX636873). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CDK4 antibody [HL1407] (GTX636873). Be the first to share your experience with this product.
![Anti-CDK4 antibody [HL1407] used in Western Blot (WB). GTX636873](/upload/website/prouct_img/small/GTX636873/GTX636873_44669_20220715_WB_shRNA_watermark_22071823_811.webp "Anti-CDK4 antibody [HL1407] used in Western Blot (WB). GTX636873")
![Anti-CDK4 antibody [HL1407] used in IHC (Paraffin sections) (IHC-P). GTX636873](/upload/website/prouct_img/small/GTX636873/GTX636873_44669_20220603_IHC-P_M_22062919_233.webp "Anti-CDK4 antibody [HL1407] used in IHC (Paraffin sections) (IHC-P). GTX636873")
![Anti-CDK4 antibody [HL1407] used in Western Blot (WB). GTX636873](/upload/website/prouct_img/small/GTX636873/GTX636873_44669_20220513_WB_w_23061202_579.webp "Anti-CDK4 antibody [HL1407] used in Western Blot (WB). GTX636873")
![Anti-CDK4 antibody [HL1407] used in Western Blot (WB). GTX636873](/upload/website/prouct_img/small/GTX636873/GTX636873_T-44620_20220318_WB_M_R_w_23061202_998.webp "Anti-CDK4 antibody [HL1407] used in Western Blot (WB). GTX636873")
![Anti-CDK4 antibody [HL1407] used in Western Blot (WB). GTX636873](/upload/website/prouct_img/normal/GTX636873/GTX636873_44669_20220715_WB_shRNA_watermark_22071823_811.webp "Anti-CDK4 antibody [HL1407] used in Western Blot (WB). GTX636873")
![Anti-CDK4 antibody [HL1407] used in IHC (Paraffin sections) (IHC-P). GTX636873](/upload/website/prouct_img/normal/GTX636873/GTX636873_44669_20220603_IHC-P_M_22062919_233.webp "Anti-CDK4 antibody [HL1407] used in IHC (Paraffin sections) (IHC-P). GTX636873")
![Anti-CDK4 antibody [HL1407] used in Western Blot (WB). GTX636873](/upload/website/prouct_img/normal/GTX636873/GTX636873_44669_20220513_WB_w_23061202_579.webp "Anti-CDK4 antibody [HL1407] used in Western Blot (WB). GTX636873")
![Anti-CDK4 antibody [HL1407] used in Western Blot (WB). GTX636873](/upload/website/prouct_img/normal/GTX636873/GTX636873_T-44620_20220318_WB_M_R_w_23061202_998.webp "Anti-CDK4 antibody [HL1407] used in Western Blot (WB). GTX636873")