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CDK4 antibody

KO/KD Orthogonal
Anti-CDK4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102993
Anti-CDK4 antibody used in Western Blot (WB). GTX102993
Anti-CDK4 antibody used in Western Blot (WB). GTX102993
Anti-CDK4 antibody used in Western Blot (WB). GTX102993
Anti-CDK4 antibody used in IHC (Paraffin sections) (IHC-P). GTX102993
Anti-CDK4 antibody used in Western Blot (WB). GTX102993
Anti-CDK4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102993
Anti-CDK4 antibody used in Western Blot (WB). GTX102993
Anti-CDK4 antibody used in Western Blot (WB). GTX102993
Anti-CDK4 antibody used in Western Blot (WB). GTX102993
Anti-CDK4 antibody used in Western Blot (WB). GTX102993

Cat. No. GTX102993

Reference 24
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Bovine
Package
100 μl ($399),
25 μl ($169)
See all CDK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

PC-12 , 293T

Predict Reactivity

Sheep, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDK4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949951

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 4 , CMM3 , PSK-J3

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDK4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102993

GTX102993 ICC/IF Image

CDK4 antibody detects CDK4 protein at nucleus by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDK4 protein stained by CDK4 antibody (GTX102993) diluted at 1:1000.
Red: p21 Cip1, a nucleus marker, stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-CDK4 antibody used in Western Blot (WB). GTX102993

GTX102993 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (GTX102993) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDK4 antibody used in Western Blot (WB). GTX102993

GTX102993 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (GTX102993) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDK4 antibody used in Western Blot (WB). GTX102993

GTX102993 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (GTX102993) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDK4 antibody used in IHC (Paraffin sections) (IHC-P). GTX102993

GTX102993 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using CDK4(GTX102993) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CDK4 antibody used in Western Blot (WB). GTX102993

GTX102993 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (GTX102993) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDK4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102993

GTX102993 ICC/IF Image

CDK4 antibody detects CDK4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDK4 protein stained by CDK4 antibody (GTX102993) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-CDK4 antibody used in Western Blot (WB). GTX102993

GTX102993 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (GTX102993) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDK4 antibody used in Western Blot (WB). GTX102993

GTX102993 WB Image

Orthogonal

The data was published in the journal Cell Death Dis in 2014.PMID: 25429617

Anti-CDK4 antibody used in Western Blot (WB). GTX102993

GTX102993 WB Image

Orthogonal

The data was published in the journal Cell Death Dis in 2014.PMID: 25429617

Anti-CDK4 antibody used in Western Blot (WB). GTX102993

GTX102993 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28134285

REFERENCE

Application Reference

Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Chen CY et al. Evid Based Complement Alternat Med 2022; 2022 : 7033127 Antrodia salmonea Extracts Regulate p53-AR Signaling and Apoptosis in Human Prostate Cancer LNCaP Cells.
Application : WB
Reactivity : Human
Yang Q et al. Acta Pharm Sin B 2022; 12 (7) : 3139-3155 Engineering prodrug nanomicelles as pyroptosis inducer for codelivery of PI3K/mTOR and CDK inhibitors to enhance antitumor immunity.
Application : WB
Reactivity : Mouse
San-Yuan Chen et al. Oncol Lett 2022; 23 (2) : 61
Application : WB
Reactivity : Human
Elena Navarro-Guerrero et al. Sci Rep 2021; 11 (1) : 4245 Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

CDK4 antibody Cat. No. GTX102993

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Anti-CDK4 antibody used in Western Blot (WB). GTX102993
Date : Anonymous submitted on 29-Jun-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells with/without siCDK4 treatment
Amount used : 20μg
Blocking : 0.1% skim milk, 24°C, 1Hr
Primary Antibody : 1:1000, °C, Hr
Anti-CDK4 antibody used in Western Blot (WB). GTX102993
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM DOX treatment for 2 h.
Anti-CDK4 antibody used in Western Blot (WB). GTX102993
Date : Anonymous submitted on 28-Jul-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : HPAF2 cells
Amount used : 80 μg
Blocking : 5% non-fat milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Chen CY et al. Evid Based Complement Alternat Med 2022; 2022:7033127 Antrodia salmonea Extracts Regulate p53-AR Signaling and Apoptosis in Human Prostate Cancer LNCaP Cells.
Application : WB
Reactivity : Human
Yang Q et al. Acta Pharm Sin B 2022; 12 (7):3139-3155 Engineering prodrug nanomicelles as pyroptosis inducer for codelivery of PI3K/mTOR and CDK inhibitors to enhance antitumor immunity.
Application : WB
Reactivity : Mouse
San-Yuan Chen et al. Oncol Lett 2022; 23 (2):61
Application : WB
Reactivity : Human
Elena Navarro-Guerrero et al. Sci Rep 2021; 11 (1):4245 Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages.
Application : WB
Reactivity : Human
Huang HY et al. J Cell Mol Med 2020; Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis.
Application : WB
Reactivity : Human
Huang CW et al. FASEB J 2020; 34 (7):9678-9693 Irisin, an exercise myokine, potently suppresses tumor proliferation, invasion, and growth in glioma.
Application : WB
Reactivity : Human
Yeh HT et al. Eur J Pharmacol 2019; 863:172658 Flavopereirine induces cell cycle arrest and apoptosis via the AKT/p38 MAPK/ERK1/2 signaling pathway in human breast cancer cells.
Application : WB
Reactivity : Human
Chen SY et al. Int J Mol Med 2019; (Epub) Piperlongumine induces autophagy in biliary cancer cells via?reactive oxygen species-activated Erk signaling pathway.
Fararjeh AS et al. Cancers (Basel) 2019; 11 (4) Proteasome 26S Subunit, non-ATPase 3 (PSMD3) Regulates Breast Cancer by Stabilizing HER2 from Degradation.
Application : WB
Reactivity : Human
Liu YH et al. Phytomedicine 2018; 46:184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
Lu WJ et al. Food Funct 2018; 9 (8):4500-4507 Licochalcone A attenuates glioma cell growth in vitro and in vivo through cell cycle arrest.
Application : WB
Reactivity : Human
Li YJ et al. Nucleic Acids Res 2018; (Epub) Regulation of miR-34b/c-targeted gene expression program by SUMOylation.
Soyoung I. et al. Int J Clin Exp Pathol 2018; 11 (4):2246-2255 Application of adipocyte-related antibodies in undifferentiated sarcomas to identify dedifferentiated liposarcomas based on histological and clinical analysis.
Application : IHC-P
Reactivity : Human
Amulic B et al. Dev Cell 2017; 43 (4):449-462.e5 Cell-Cycle Proteins Control Production of Neutrophil Extracellular Traps.
Application : WB
Reactivity : Human
Yuan SY et al. Oncol Rep 2017; 37 (6):3606-3616 * induces apoptosis via the death receptor 5-dependent and mitochondrial-mediated pathways in human bladder cancer cells.
Application : WB
Reactivity : Human
Hsu SC et al. J Virol 2017; (Epub) Serine 235 Is the Primary NS5A Hyper-Phosphorylation Site Responsible for HCV Replication.
Liu YH et al. Sci Rep 2017; 7:41437 Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.
Application : WB
Reactivity : Human
Totty ML et al. Mol Cell Endocrinol 2016; 440:25-33 Fibroblast growth factor 9 (FGF9) regulation of cyclin D1 and cyclin-dependent kinase-4 in ovarian granulosa and theca cells of cattle.
Application : WB
Reactivity : Bovine
Chung CL et al. Evid Based Complement Alternat Med 2015; 2015:595824 Hinokitiol Negatively Regulates Immune Responses through Cell Cycle Arrest in Concanavalin A-Activated Lymphocytes.
Application : WB
Reactivity : Mouse
Parajuli KR et al. Am J Clin Exp Urol 2014; 2 (4):300-12 Methoxyacetic acid suppresses prostate cancer cell growth by inducing growth arrest and apoptosis.
Application : WB
Reactivity : Human
Chang HY et al. Cell Death Dis 2014; 5:e1540 Combination therapy targeting ectopic ATP synthase and 26S proteasome induces ER stress in breast cancer cells.
Application : WB
Reactivity : Human
Hsiao CJ et al. J Nat Prod 2014; Cephalochromin Induces G0/G1 Cell Cycle Arrest and Apoptosis in A549 Human Non-Small-Cell Lung Cancer Cells by Inflicting Mitochondrial Disruption.
Application : WB
Reactivity : Human
Huang AM et al. Biochem Pharmacol 2011; 82 (1):35-42 ube2m-mediated p27(kip1) degradation in * cytotoxicity.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169