GeneTex
United States (US)

CDK4 antibody

Cat No. GTX16516

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 33 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

34 kDa. ( Note )

Positive Control

HeLa cell lysate
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.08% Sodium Azide. Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TrpE fusion protein containing C-terminal 164 amino acids of Cdk4 (Human).

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin Dependent Kinase 4,Cmm3,Psk-J3,Cdk4

Background

Cdk4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk4 is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1/S phase, which is controlled by the regulatory subunits D type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). The mutations in this gene as well as its related proteins including D type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Two alternatively spliced variants, and multiple polyadenylation sites of this gene have been reported.

Database

Research Area

Package List Price ($)
$ 329