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CDK4 (phospho Thr172) antibody

Orthogonal
Anti-CDK4 (phospho Thr172) antibody used in Western Blot (WB). GTX00778

Cat. No. GTX00778

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all CDK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic phosphorylated peptide around T172 of human CDK4 (NP_000066.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 4 , CMM3 , PSK-J3

Cellular Localization

Cytoplasm,Nucleus,Membrane

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CDK4 (phospho Thr172) antibody used in Western Blot (WB). GTX00778

GTX00778 WB Image

Orthogonal

WB analysis of NIH/3T3 cells treated by Hydroxyurea (4 mM) at 37ºC for 20 hours using GTX00778 CDK4 (phospho Thr172) antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Bo-Ruei Chen et al. Elife 2021; 10 LIN37-DREAM prevents DNA end resection and homologous recombination at DNA double-strand breaks in quiescent cells.
Application : WB
Reactivity : Mouse
Fu H et al. Mol Med Rep 2021; 23 (1) CSN1 facilitates proliferation and migration of hepatocellular carcinoma cells by upregulating cyclin A2 expression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399