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CDK5 antibody [2H6]

KO/KD Overexpression
Anti-CDK5 antibody [2H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84696
Anti-CDK5 antibody [2H6] used in Western Blot (WB). GTX84696
Anti-CDK5 antibody [2H6] used in Western Blot (WB). GTX84696
Anti-CDK5 antibody [2H6] used in Western Blot (WB). GTX84696
Anti-CDK5 antibody [2H6] used in Western Blot (WB). GTX84696
Anti-CDK5 antibody [2H6] used in Flow cytometry (FACS). GTX84696
Anti-CDK5 antibody [2H6] used in Western Blot (WB). GTX84696
Anti-CDK5 antibody [2H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84696

Cat. No. GTX84696

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2H6

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Dog
Package
100 μl ($519)
See all CDK5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:50-1:100
FACS 1:100
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CDK5 (NP_004926) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 5 , LIS7 , PSSALRE

Background

Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.

Database

Research Area

DATA IMAGES

Anti-CDK5 antibody [2H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84696

GTX84696 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CDK5 plasmid using GTX84696 CDK5 antibody [2H6].

Anti-CDK5 antibody [2H6] used in Western Blot (WB). GTX84696

GTX84696 WB Image

KO/KD

WB analysis of wild type and CDK5 knock out 293T cell lysate(10μg per lane) using GTX84696 CDK5 antibody [2H6].
Dilution : 1:500

Anti-CDK5 antibody [2H6] used in Western Blot (WB). GTX84696

GTX84696 WB Image

WB analysis of various cell lines using GTX84696 CDK5 antibody [2H6].
Loading : 35 ug per lane

Anti-CDK5 antibody [2H6] used in Western Blot (WB). GTX84696

GTX84696 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84696 CDK5 antibody [2H6].
Loading : 10 ug per lane
Dilution : 1:200

Anti-CDK5 antibody [2H6] used in Western Blot (WB). GTX84696

GTX84696 WB Image

WB analysis of various samples using GTX84696 CDK5 antibody [2H6].
Loading : 10 ug per lane
Dilution : 1:200

Anti-CDK5 antibody [2H6] used in Flow cytometry (FACS). GTX84696

GTX84696 FACS Image

FACS analysis of HEK293T cells transfected with either CDK5 plasmid(Red) or empty vector control plasmid(Blue) using GTX84696 CDK5 antibody [2H6].

Anti-CDK5 antibody [2H6] used in Western Blot (WB). GTX84696

GTX84696 WB Image

Overexpression

WB analysis of HEK293T cells transfected with CDK5 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84696 CDK5 antibody [2H6].
Loading : 5 ug per lane

Anti-CDK5 antibody [2H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84696

GTX84696 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX84696 CDK5 antibody [2H6].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519