APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , Jurkat , 293T
Predict Reactivity
Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CDK5. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036536
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
cyclin dependent kinase 5 , LIS7 , PSSALRE
Cellular Localization
Cytoplasm , Cell projection , lamellipodium , growth cone
Background
Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
Database
Research Area
DATA IMAGES
![Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328](/templates/default/js/lightbox/images/loading.gif "Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328")
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GTX108328 WB Image
Wild-type (WT) and CDK5 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody [C2C3], C-term (GTX108328) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX108328 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody (GTX108328) diluted at 1:500.
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GTX108328 WB Image
Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX108328 diluted at 1:1000
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GTX108328 IHC-P Image
Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CDK5(GTX108328) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX108328 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A549, using CDK5(GTX108328) antibody at 1:200 dilution.
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GTX108328 IHC-P Image
CDK5 antibody [C2C3], C-term detects CDK5 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
CDK5 antibody [C2C3], C-term (GTX108328) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
REVIEW
There are currently no reviews for CDK5 antibody [C2C3], C-term (GTX108328). Be the first to share your experience with this product.
![Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328](/upload/website/prouct_img/small/GTX108328/GTX108328_39750_20180209_WB_KO_watermark_19011507_455.jpg "Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328")
![Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328](/upload/website/prouct_img/small/GTX108328/GTX108328_39750_20160331_WB_19011507_863.jpg "Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328")
![Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328](/upload/website/prouct_img/small/GTX108328/GTX108328_39750_WB_M_brain_19011507_729.jpg "Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328")
![Anti-CDK5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108328](/upload/website/prouct_img/small/GTX108328/GTX108328_39750_IHC_19011507_647.jpg "Anti-CDK5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108328")
![Anti-CDK5 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108328](/upload/website/prouct_img/small/GTX108328/GTX108328_IFA_19011507_136.jpg "Anti-CDK5 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108328")
![Anti-CDK5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108328](/upload/website/prouct_img/small/GTX108328/GTX108328_39750_IHC_R_19011507_281.jpg "Anti-CDK5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108328")
![Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328](/upload/website/prouct_img/normal/GTX108328/GTX108328_39750_20180209_WB_KO_watermark_19011507_455.jpg "Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328")
![Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328](/upload/website/prouct_img/normal/GTX108328/GTX108328_39750_20160331_WB_19011507_863.jpg "Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328")
![Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328](/upload/website/prouct_img/normal/GTX108328/GTX108328_39750_WB_M_brain_19011507_729.jpg "Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328")
![Anti-CDK5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108328](/upload/website/prouct_img/normal/GTX108328/GTX108328_39750_IHC_19011507_647.jpg "Anti-CDK5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108328")
![Anti-CDK5 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108328](/upload/website/prouct_img/normal/GTX108328/GTX108328_IFA_19011507_136.jpg "Anti-CDK5 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108328")
![Anti-CDK5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108328](/upload/website/prouct_img/normal/GTX108328/GTX108328_39750_IHC_R_19011507_281.jpg "Anti-CDK5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108328")