GeneTex
United States (US)

CDK5 antibody [C2C3], C-term

GTX108328_39750_20180209_WB_KO_watermark_19011507_455.jpg
GTX108328_39750_20160331_WB_19011507_863.jpg
GTX108328_39750_WB_M_brain_19011507_729.jpg
GTX108328_39750_IHC_19011507_647.jpg
GTX108328_IFA_19011507_136.jpg
GTX108328_39750_IHC_R_19011507_281.jpg

Cat No. GTX108328

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , Jurkat , Mouse brain

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CDK5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin Dependent Kinase 5,Lis7,Pssalre,Cdk5

Cellular Localization

Cytoplasm , Cell projection , lamellipodium , growth cone

Background

Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.

Database

Research Area

DATA IMAGES
GTX108328_39750_20180209_WB_KO_watermark_19011507_455.jpg

GTX108328 WB Image

Wild-type (WT) and CDK5 knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody [C2C3], C-term (GTX108328) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX108328_39750_20160331_WB_19011507_863.jpg

GTX108328 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody (GTX108328) diluted at 1:500.

GTX108328_39750_WB_M_brain_19011507_729.jpg

GTX108328 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX108328 diluted at 1:1000

GTX108328_39750_IHC_19011507_647.jpg

GTX108328 IHC-P Image

Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CDK5(GTX108328) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108328_IFA_19011507_136.jpg

GTX108328 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using CDK5(GTX108328) antibody at 1:200 dilution.

GTX108328_39750_IHC_R_19011507_281.jpg

GTX108328 IHC-P Image

CDK5 antibody [C2C3], C-term detects CDK5 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
CDK5 antibody [C2C3], C-term (GTX108328) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169