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CDK5 antibody [C2C3], C-term

Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328
Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328
Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328
Anti-CDK5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108328
Anti-CDK5 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108328
Anti-CDK5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108328

Cat No. GTX108328

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , Jurkat , Mouse brain

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CDK5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036536

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin dependent kinase 5 , LIS7 , PSSALRE

Cellular Localization

Cytoplasm , Cell projection , lamellipodium , growth cone

Background

Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.

Database

Research Area

DATA IMAGES

Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328

GTX108328 WB Image

Wild-type (WT) and CDK5 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody [C2C3], C-term (GTX108328) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328

GTX108328 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody (GTX108328) diluted at 1:500.

Anti-CDK5 antibody [C2C3], C-term used in Western Blot (WB). GTX108328

GTX108328 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX108328 diluted at 1:1000

Anti-CDK5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108328

GTX108328 IHC-P Image

Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CDK5(GTX108328) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CDK5 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108328

GTX108328 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using CDK5(GTX108328) antibody at 1:200 dilution.

Anti-CDK5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108328

GTX108328 IHC-P Image

CDK5 antibody [C2C3], C-term detects CDK5 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
CDK5 antibody [C2C3], C-term (GTX108328) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169