GeneTex
United States (US)

CDK5 (phospho Tyr15) antibody

GTX32375_20190131_ICCIF_19013101_828.jpg
GTX32375_20190131_IHC-P_19013101_175.jpg
GTX32375_20190131_WB_19013101_255.jpg

Cat No. GTX32375

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

33 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of CDK5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin Dependent Kinase 5,Lis7,Pssalre,Cdk5

Cellular Localization

Cell junction,Cell membrane,Cell projection,Cytoplasm,Growth cone,Lamellipodium,Nucleus,Perikaryon,Postsynaptic cell membrane,Postsynaptic density,Synapse

Background

This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]

Database

Research Area

DATA IMAGES
GTX32375_20190131_ICCIF_19013101_828.jpg

GTX32375 ICC/IF Image

ICC/IF analysis of formalin-fixed HEK293T cells using GTX32375 CDK5 (phospho Tyr15) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32375_20190131_IHC-P_19013101_175.jpg

GTX32375 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32375 CDK5 (phospho Tyr15) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX32375_20190131_WB_19013101_255.jpg

GTX32375 WB Image

WB analysis of PMA-treated HT29 (A), PMA-treated HEK293 (B) whole cell lysates using GTX32375 CDK5 (phospho Tyr15) antibody.

Package List Price ($)
$ 319