CDK5RAP1 antibody

Cat. No. GTX111910

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Rat
Package	100 μl ($419), 25 μl ($169)

See all CDK5RAP1 products

Datasheet
Document

View product citations for antibody GTX111910 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDK5RAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166141

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CDK5 regulatory subunit associated protein 1 , C20orf34 , C42 , CGI-05 , HSPC167

Background

Neuronal CDC2-like kinase, which is involved in the regulation of neuronal differentiation, is composed of a catalytic subunit, CDK5, and an activating subunit, p25NCK5A. The protein encoded by this gene binds to p25NCK5A and therefore may be involved in neuronal differentiation. Multiple transcript variants exist for this gene, but the full-length natures of only two have been determined. [provided by RefSeq]

Database

Gene ID: 51654 CDK5RAP1
UniProt: Q96SZ6 CDK5RAP1

Research Area

DATA IMAGES

GTX111910 IHC-P Image

CDK5RAP1 antibody detects CDK5RAP1 protein at cytoplasm in Rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded Rat brain.
CDK5RAP1 antibody (GTX111910) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111910 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDK5RAP1 antibody (GTX111910) diluted at 1:500.
The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.
Reference: PMID: 22415164

GTX111910 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CDK5RAP1(GTX111910) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 419

25 μl

$ 169

Qty