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CDK6 (Phospho Tyr13) antibody

Orthogonal
Anti-CDK6 (Phospho Tyr13) antibody used in IHC (Paraffin sections) (IHC-P). GTX50352
Anti-CDK6 (Phospho Tyr13) antibody used in Western Blot (WB). GTX50352
Anti-CDK6 (Phospho Tyr13) antibody used in Western Blot (WB). GTX50352
Anti-CDK6 (Phospho Tyr13) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50352

Cat. No. GTX50352

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all CDK6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 13 (Q-Q-Y(p)-E-C) derived from human CDK6.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 6 , MCPH12 , PLSTIRE

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-CDK6 (Phospho Tyr13) antibody used in IHC (Paraffin sections) (IHC-P). GTX50352

GTX50352 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50352 CDK6 (Phospho Tyr13) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-CDK6 (Phospho Tyr13) antibody used in Western Blot (WB). GTX50352

GTX50352 WB Image

Orthogonal

WB analysis of extracts from 293 cells untreated or treated with HU using GTX50352 CDK6 (Phospho Tyr13) antibody.

Anti-CDK6 (Phospho Tyr13) antibody used in Western Blot (WB). GTX50352

GTX50352 WB Image

Orthogonal

WB analysis of extracts from HUVEC cells treated with calf intestinal phosphatase (CIP) using GTX50352 CDK6 (Phospho Tyr13) antibody.

Anti-CDK6 (Phospho Tyr13) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50352

GTX50352 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50352 CDK6 (Phospho Tyr13) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399